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Investigating Flagella-Driven Motility in Escherichia coli by Applying Three Established Techniques in a Series
JoVE Journal
Biology
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JoVE Journal Biology
Investigating Flagella-Driven Motility in Escherichia coli by Applying Three Established Techniques in a Series
DOI:

07:59 min

May 10, 2020

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Chapters

  • 00:05Introduction
  • 01:03Culture Preparation
  • 02:22Quantifying Flagella Motor Behavior via Cell Tethering
  • 04:55Preparation of Swarms in a Border-crossing Assay
  • 06:03Results: Motility of yhjH ycgR E. coli Strain
  • 07:20Conclusion

Summary

Automatic Translation

Many bacteria use flagella-driven motility to navigate their environment and colonize favorable surroundings both individually and as a collective. Demonstrated here is the use of three established methods that exploit motility as a selection tool to identify components/pathways contributing to swimming and swarming motility.

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