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Brug af primære kulturperler Hippocampal Neuroner til at studere forsamlingen af Axon indledende segmenter
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Neuroscience
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JoVE Journal Neuroscience
Use of Primary Cultured Hippocampal Neurons to Study the Assembly of Axon Initial Segments

Brug af primære kulturperler Hippocampal Neuroner til at studere forsamlingen af Axon indledende segmenter

DOI:
10.3791/61411-v

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06:53 min

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February 12, 2021

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,
1School of Basic Medical Sciences,Xi’an Jiaotong University, 2Department of Cell Biology,Duke University, 3Department of Biochemistry,Duke University

Chapters

  • 00:04Introduction
  • 00:24Culture Hippocampal Neurons
  • 02:37Disruption of AIS by Knockout of Ankyrin-G at an Early Stage of Neuron Development
  • 04:17Quantification of Axon Initial Segment
  • 04:58Results: A Full Rescue of AIS by 480kDa AnkG in ANK3-E22/23-flox Neurons Transfected with Cre

Summary

Automatic Translation

Automatic Translation

Her beskrev vi en protokol til kvantitativt at studere samlingen og strukturen af axon indledende segmenter (AIS) af hippocampal neuroner, der mangler formonteret AIS på grund af fraværet af en kæmpe ankyrin-G.

Tags

Primary Cultured Hippocampal NeuronsAxon Initial SegmentsAnkyrin-GCRE BFP DNAAnkyrin-G GFP DNATransfectionDissectionTrypsin DigestionTriturationNeuronal CultureGlial Cell Feeder Dishes

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