Journal
/
/
Brug af primære kulturperler Hippocampal Neuroner til at studere forsamlingen af Axon indledende segmenter
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Use of Primary Cultured Hippocampal Neurons to Study the Assembly of Axon Initial Segments
DOI:

06:53 min

February 12, 2021

,

Chapters

  • 00:04Introduction
  • 00:24Culture Hippocampal Neurons
  • 02:37Disruption of AIS by Knockout of Ankyrin-G at an Early Stage of Neuron Development
  • 04:17Quantification of Axon Initial Segment
  • 04:58Results: A Full Rescue of AIS by 480kDa AnkG in ANK3-E22/23-flox Neurons Transfected with Cre

Summary

Automatic Translation

Her beskrev vi en protokol til kvantitativt at studere samlingen og strukturen af axon indledende segmenter (AIS) af hippocampal neuroner, der mangler formonteret AIS på grund af fraværet af en kæmpe ankyrin-G.

Related Videos

Read Article