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Journal / Bioengineering / Manipulation of Single Neural Stem Cells and…
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Bioengineering

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JoVE Journal Bioengineering
Manipulation of Single Neural Stem Cells and Neurons in Brain Slices using Robotic Microinjection
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Manipulation of Single Neural Stem Cells and Neurons in Brain Slices using Robotic Microinjection

Article DOI: 10.3791/61599-v • 11:40 min • January 21st, 2021
January 21st, 2021 •
*1,2, *3, 3, 3,4, 1,5,6
1Department of Biomedical Engineering, University of Minnesota, 2Department of Biomedical Engineering, Duke University, 3Max Planck Institute of Molecular Cell Biology and Genetics, 4Max Planck Institute for Evolutionary Anthropology, 5Department of Mechanical Engineering, University of Minnesota, 6Graduate Program in Neuroscience, University of Minnesota
* These authors contributed equally

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Summary
Automatic Translation

This protocol demonstrates the use of a robotic platform for microinjection into single neural stem cells and neurons in brain slices. This technique is versatile and offers a method of tracking cells in tissue with high spatial resolution.

Tags

Neural Stem Cells Neurons Brain Slices Robotic Microinjection Tissue Formation Development Visual Demonstration Protocol Living Tissue Single Cells Auto-injector Software Microinjection Pipettes Borosilicate Glass Capillaries Agarose Fluorescent Tracer Centrifuge
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