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JoVE Journal
Biology

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FLIM-FRET Measurements of Protein-Protein Interactions in Live Bacteria.
 

FLIM-FRET Measurements of Protein-Protein Interactions in Live Bacteria.

Article DOI: 10.3791/61602
August 25th, 2020

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Summary August 25th, 2020

We describe here a protocol to characterize protein-protein interactions between two highly-differently expressed proteins in live Pseudomonas aeruginosa using FLIM-FRET measurements. The protocol includes bacteria strain constructions, bacteria immobilization, imaging and post-imaging data analysis routines.

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