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使用CRISPR/Cas9的人类B细胞基因组工程
JoVE Journal
Biology
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JoVE Journal
Biology
Genome Engineering of Primary Human B Cells Using CRISPR/Cas9
Please note that all translations are automatically generated.
Click here for the English version.
使用CRISPR/Cas9的人类B细胞基因组工程
DOI:
10.3791/61855-v
•
08:20 min
•
November 03, 2020
•
Kanut Laoharawee
2,3
,
Matthew J. Johnson
2,3
,
Walker S. Lahr
2,3
,
Joseph J. Peterson
2,3
,
Beau R. Webber
2,3
,
Branden S. Moriarity
2,3
1
Department of Pediatrics
,
University of Minnesota
,
2
Center for Genomic Engineering
,
University of Minnesota
,
3
Masonic Cancer Center
,
University of Minnesota
Chapters
00:04
Introduction
00:56
Human B-cell Expansion
02:25
Primary Human B-cell Engineering
05:30
Results: CRISPR/Cas9-based Genome Engineering of Primary Human B Cells for Gene Knockout and Knock-in
07:33
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们为CRISPR/Cas9基础基因组工程提供详细的分步协议,用于基因敲除(KO)和敲击(KI),以研究B细胞中基因的生物功能和B细胞治疗的开发。
Tags
Genome Engineering
CRISPR/Cas9
Primary Human B Cells
Gene Knockout
Gene Insertion
Recombinant Antibody
Enzymopathy
Cell Culture
Electroporation
Transfection
SgRNA
Cas9 MRNA
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