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Journal / Biology / 使用CRISPR/Cas9的人类B细胞基因组工程
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使用CRISPR/Cas9的人类B细胞基因组工程
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使用CRISPR/Cas9的人类B细胞基因组工程

Article DOI: 10.3791/61855-v • 08:20 min • November 3rd, 2020
November 3rd, 2020 •
1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3
1Department of Pediatrics, University of Minnesota, 2Center for Genomic Engineering, University of Minnesota, 3Masonic Cancer Center, University of Minnesota

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在这里，我们为CRISPR/Cas9基础基因组工程提供详细的分步协议，用于基因敲除（KO）和敲击（KI），以研究B细胞中基因的生物功能和B细胞治疗的开发。

Tags

生物学，第165期，CRISPR/Cas9，基因组工程，重组AAV，基因编辑，人类B细胞
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