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JoVE Journal
Biochemistry
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Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening
 

Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening

Article doi: 10.3791/61894
January 16th, 2021

Summary January 16th, 2021

This article presents a method to generate protein crystals derivatized with I3C (5-amino-2,4,6-triiodoisophthalic acid) using microseeding to generate new crystallization conditions in sparse matrix screens. The trays can be set up using liquid dispensing robots or by hand.

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