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Journal / Biochemistry / Derivatization of Protein Crystals with I3C using …
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Biochemistry
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JoVE Journal Biochemistry
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Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening

Article DOI: 10.3791/61894-v • 14:04 min • January 16th, 2021
January 16th, 2021 •
*1, *1,2, 1,2, 1
1School of Biological Sciences, The University of Adelaide, 2Institute of Photonics and Advanced Sensing (IPAS), School of Biological Sciences, The University of Adelaide
* These authors contributed equally

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Summary
Automatic Translation

This article presents a method to generate protein crystals derivatized with I3C (5-amino-2,4,6-triiodoisophthalic acid) using microseeding to generate new crystallization conditions in sparse matrix screens. The trays can be set up using liquid dispensing robots or by hand.

Tags

Derivation Protein Crystals I3C Random Microseed Matrix Screening Crystallization Protocol X-ray Crystallography Phasing Molecule X-ray Crystal Structure Heavy Atoms Derivatized Crystal X-ray Diffraction Data Computational Solution Optimized Screening Procedure Microseeds Crystal Growth Independent Conditions Anomalous Signal Single Wavelength Anomalous Dispersion Phasing
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