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Building a Simple and Versatile Illumination System for Optogenetic Experiments
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Building a Simple and Versatile Illumination System for Optogenetic Experiments

Building a Simple and Versatile Illumination System for Optogenetic Experiments

DOI:
10.3791/61914-v

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06:41 min

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January 12, 2021

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, , , , , , , , , ,
1Department of Bioengineering,University of California, San Diego, 2University of California, San Diego, 3Department of Mathematical Computational, and Systems biology,University of California, Irvine, 4Department of Biomedical Engineering,Duke University

Chapters

  • 00:05Introduction
  • 00:31Electrical Circuit Construction
  • 01:38Light Emitting Diode (LED) Construction
  • 05:10Results: Representative LED-Controlled Phytochrome B (PhyB) Expression Analysis
  • 06:06Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes how to perform optogenetic experiments for controlling gene expression with red and far-red light using PhyB and PIF3. Included are step-by-step instructions for building a simple and flexible illumination system, which enables the control of gene expression or other optogenetics with a computer.

Tags

Keywords: Illumination SystemOptogenetic ExperimentsLEDElectrical CircuitArduinoSolderingWire ConnectorResistorCathodeAnodeSolder PasteShrink TubeFlux

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