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JoVE Journal
Bioengineering

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Simple, Affordable, and Modular Patterning of Cells using DNA
 

Simple, Affordable, and Modular Patterning of Cells using DNA

Article DOI: 10.3791/61937-v
February 24th, 2021

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Summary February 24th, 2021

Here we present a protocol to micropattern cells at single-cell resolution using DNA-programmed adhesion. This protocol uses a benchtop photolithography platform to create patterns of DNA oligonucleotides on a glass slide and then labels cell membranes with commercially available complementary oligonucleotides. Hybridization of the oligos results in programmed cell adhesion.

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