Microtissue Arrays
Microtissue Arrays - Related Videos
11:28
3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening
0 Views •
Cited by 7 •
2017
This protocol describes the fabrication of elastic 3D macroporous microcryogels by integrating microfabrication with cryogelation technology. Upon loading with cells, 3D microtissues are generated, which can be readily injected in vivo to facilitate regenerative therapy or assembled into arrays for in vitro high-throughput drug screening.
10:37
Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells
0 Views •
Cited by 18 •
2021
Here, we describe an easy-to-use methodology to generate 3D self-assembled cardiac microtissue arrays composed of pre-differentiated human-induced pluripotent stem cell-derived cardiomyocytes, cardiac fibroblasts, and endothelial cells. This user-friendly and low cell requiring technique to generate cardiac microtissues can be implemented for disease modeling and early stages of drug development.
09:16
Array Comparative Genomic Hybridization (Array CGH) for Detection of Genomic Copy Number Variants
0 Views •
Cited by 7 •
2015
Array CGH for the detection of genomic copy number variants has replaced G-banded karyotype analysis. This paper describes the technology and its application in a diagnostic service laboratory.
09:56
Layer-by-layer Collagen Deposition in Microfluidic Devices for Microtissue Stabilization
0 Views •
Cited by 7 •
2015
The creation of functional microtissues within microfluidic devices requires the stabilization of cell phenotypes by adapting traditional cell culture techniques to the limited spatial dimensions in microdevices. Modification of collagen allows the layer-by-layer deposition of ultrathin collagen assemblies that can stabilize primary cells, such as hepatocytes, as microfluidic tissue models.
19:40
Design and Use of Multiplexed Chemostat Arrays
0 Views •
Cited by 108 •
2013
We developed and validated a small-footprint array of miniature chemostats built from readily available parts for low cost. Physiological and experimental evolution results were similar to larger volume chemostats. The ministat array provides a compact, inexpensive, and accessible platform for traditional chemostat experiments, functional genomics, and chemical screening applications.
FAQs
Controlling aggregate size and cellular composition helps researchers compare tissues under more consistent starting conditions. Uniform dimensions can make differences in tissue behavior, cell-cell communication, or drug response easier to attribute to the experimental variable rather than uncontrolled variation among samples. This standardization is especially valuable when many microtissues are analyzed in parallel.
Three-dimensional organization allows cells to interact with one another and with extracellular matrix in a tissue-like arrangement. That organization can produce behavior more physiologically relevant than flat culture systems, which may not reproduce the same spatial relationships. As a result, arrays can support bioengineering studies of tissue formation, disease-related changes, and responses to candidate treatments.
These materials and structures confine cells within defined locations, creating the spatial organization needed for reproducible microtissue formation. Their use also helps regulate aggregate size, composition, and extracellular matrix interactions. Selecting an appropriate confinement format therefore affects how consistently tissues self-assemble and how reliably researchers can compare conditions across an array.
A typical workflow establishes a spatially defined platform, places selected cells into its wells, patterned regions, or hydrogel environment, and allows the cells to self-assemble into microtissues. Researchers then analyze the organized aggregates across many positions under comparable conditions. This workflow supports systematic measurements while using smaller amounts of samples and reagents than less parallelized approaches.
Researchers may choose these arrays when they need parallel comparisons of drug responses or disease-related tissue changes. The controlled arrangement allows multiple microtissues to be evaluated systematically, while three-dimensional organization provides a model that can be more physiologically relevant than conventional two-dimensional culture. Their scalability also makes them suitable for high-throughput screening.
In bioengineering, the platforms support tissue formation studies, biomaterials evaluation, regenerative medicine research, and analysis of cell-cell communication. Their controlled extracellular matrix interactions help investigators examine how engineered environments influence tissue behavior. Because arrays combine reproducibility with scalable, parallel analysis, they can compare many design conditions while reducing sample and reagent requirements.