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Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels
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Chapters
- 00:04Introduction
- 00:50Scaffold Fibronectin Coating
- 01:47Endothelial Cells Seeding
- 02:57Support Cell Seeding and Co-Culture
- 05:31Results: Analysis of Organized Vascular Networks on Tessellated Scaffold Geometries
- 07:22Conclusion
Engineered tissues heavily rely on proper vascular networks to provide vital nutrients and gases and remove metabolic waste. In this work, a stepwise seeding protocol of endothelial cells and support cells creates highly organized vascular networks in a high-throughput platform for studying developing vessel behavior in a controlled 3D environment.
