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JoVE Journal
Bioengineering

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Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels
 

Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels

Article DOI: 10.3791/61995-v
January 14th, 2021

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Summary January 14th, 2021

Engineered tissues heavily rely on proper vascular networks to provide vital nutrients and gases and remove metabolic waste. In this work, a stepwise seeding protocol of endothelial cells and support cells creates highly organized vascular networks in a high-throughput platform for studying developing vessel behavior in a controlled 3D environment.

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