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Refined CLARITY-Based Tissue Clearing for Three-Dimensional Fibroblast Organization in Healthy and Injured Mouse Hearts
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JoVE Journal Medicine
Refined CLARITY-Based Tissue Clearing for Three-Dimensional Fibroblast Organization in Healthy and Injured Mouse Hearts

Refined CLARITY-Based Tissue Clearing for Three-Dimensional Fibroblast Organization in Healthy and Injured Mouse Hearts

DOI:
10.3791/62023-v

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07:10 min

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May 16, 2021

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, , ,
1Department of Molecular Genetics, Biochemistry, and Microbiology,University of Cincinnati College of Medicine, 2Division of Molecular Cardiovascular Biology,Cincinnati Children’s Hospital Medical Center, 3Confocal Imaging Core,Cincinnati Children’s Hospital Medical Center

Chapters

  • 00:05Introduction
  • 00:38Adult Mouse Heart Clearing
  • 03:583D Cleared Heart Imaging
  • 04:53Results: Representative 3D Fibroblast Organization Visualization
  • 06:35Conclusion

Summary

Automatic Translation

Automatic Translation

A refined method of tissue clearing was developed and applied to the adult mouse heart. This method was designed to clear dense, autofluorescent cardiac tissue, while maintaining labeled fibroblast fluorescence attributed to a genetic reporter strategy.

Tags

CLARITYFibroblastMouse Heart3D OrganizationInjuryFluorescenceHydrogelElectrophoresis

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