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Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
DOI:

07:25 min

January 05, 2021

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Chapters

  • 00:04Introduction
  • 00:42Cell Transfer and Culture
  • 01:21Metabolite Extraction
  • 02:44Extracted Metabolite Separation and Analysis
  • 04:04Membrane Integrity Assessment
  • 05:24Results: Representative Metabolic Intermediate and Product Identification and Quantification
  • 06:50Conclusion

Summary

Automatic Translation

We present a protocol for the identification and quantitation of major classes of water-soluble metabolites in the yeast Saccharomyces cerevisiae. The described method is versatile, robust, and sensitive. It allows the separation of structural isomers and stereoisomeric forms of water-soluble metabolites from each other.

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