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Neuroscience

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Analysis of Translation in the Developing Mouse Brain using Polysome Profiling
 

Analysis of Translation in the Developing Mouse Brain using Polysome Profiling

Article DOI: 10.3791/62088
May 22nd, 2021

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Summary May 22nd, 2021

The development of the mammalian brain requires proper control of gene expression at the level of translation. Here, we describe a polysome profiling system with an easy-to-assemble sucrose gradient-making and fractionation platform to assess the translational status of mRNAs in the developing brain.

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