Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / A 3D Cartographic Description of the Cell by Cryo …
JoVE Journal
Biology

This content is Open Access.

JoVE Journal Biology
A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography
Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids
Journal (Biology)
Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids
Feeding Assay to Evaluate the Effects of Quercetin on Helicoverpa armigera Growth and Development (Video) | JoVE
Journal (Biology)
Feeding Assay to Evaluate the Effects of Quercetin on Helicoverpa armigera Growth and Development (Video) | JoVE
 

A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography

Article DOI: 10.3791/62190-v • 08:47 min • March 15th, 2021
March 15th, 2021 •
1, 1, 1, 1, 1, 2, 2, 2, 1, 1
1MISTRAL beamline, Alba Light Source, Cerdanyola del Valles, Spain, 2Beamline B24, Diamond Light Source, Harwell Science and Innovation Campus, Didcot, UK

Chapters

Summary
Automatic Translation

Here, a protocol describing the sample preparation and data collection steps required in cryo soft X-ray tomography (SXT) to image the ultrastructure of whole cryo-preserved cells at a resolution of 25 nm half pitch, is presented.

Tags

Cryo Soft X-ray Tomography Cell Quantitative Information Imaging Techniques Frozen Hydrated State Staining Sectioning High-throughput Technique Cell Restoration Infected Cells Defective Cells Antiviral Drugs Gene Therapy Transmission X-ray Microscope Liquid Nitrogen Cryo Box Cryo Conditions Sample Holders Transfer Chamber Low Vacuum
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter