Tomography Tilt Series
Tomography Tilt Series - Related Videos
02:32
Generation of Tilted Amygdala Slices from a Mouse Brain
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2025
The video demonstrates the generation of tilted amygdala slices from a mouse brain. The brain is positioned at an angle on the vibratome specimen stage to obtain slices containing the amygdala and axons from the medial prefrontal cortex that form synaptic connections with the amygdala. The brain slices are maintained at the physiological temperature to preserve the tissue for further experiments.
03:53
Balmer Series - Concepts
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2020
The Bohr Model Niels Bohr proposed a model for the hydrogen atom in 1913 that described discrete energy states are associated with a fixed electron orbit around the nucleus. Importantly, an atom cannot discharge energy while its electrons are in stationary states. An electron can only emit energy by changing energy states. To change energy states, an electron must move from one orbit to another by either absorbing or emitting energy. This change can only occur if the absorbed or emitted energy...
03:57
Balmer Series - Student Protocol
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2020
Source: Smaa Koraym at Johns Hopkins University, MD, USA Spectral Emission Lines of Hydrogen, Helium, and NeonExpand In this experiment, you will use a spectrophotometer to measure the distinct wavelengths of light emitted in the UV and visible range by electron relaxation in hydrogen, helium, and neon. Before starting the lab, make a table in your lab notebook for the elements that you will analyze, the color of the light that you observe from the lamps, and the recorded wavelengths. Table...
08:04
Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography
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Cited by 9 •
2017
This report describes a sample preparation protocol and specific imaging conditions for performing scanning transmission electron tomography of thick biological specimens.
11:33
Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography
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Cited by 48 •
2016
We present a protocol on how to utilize high-throughput cryo-electron tomography to determine high resolution in situ structures of molecular machines. The protocol permits large amounts of data to be processed, avoids common bottlenecks and reduces resource downtime, allowing the user to focus on important biological questions.
FAQs
Consistent imaging conditions help ensure that differences among projections primarily reflect changes in viewing angle rather than changes in how the specimen was imaged. This improves the reliability of subsequent alignment and volumetric reconstruction. Maintaining comparable conditions is therefore essential when combining projections to interpret biological architecture in three dimensions.
Each viewing angle provides a different projection of the same specimen, adding structural information that is not available from one direction alone. Combining these perspectives helps resolve the spatial organization of cellular features, organelles, or macromolecular assemblies. The resulting three-dimensional interpretation can clarify structures that remain difficult to understand in a single two-dimensional image.
Alignment algorithms register the projections so that corresponding structural features are placed consistently relative to one another. Once the images are aligned, computational reconstruction can combine their information into a volumetric representation. Accurate alignment matters because the quality of the final three-dimensional structure depends on how coherently the separate views are assembled.
The specimen is imaged repeatedly while being incrementally tilted through different viewing angles, with imaging conditions kept as consistent as possible. The resulting projections are then aligned computationally, and reconstruction algorithms calculate a volume from the combined images. This workflow links controlled image acquisition with computational analysis of the specimen’s three-dimensional organization.
In biology, the approach can reveal cellular architecture, organelle organization, and macromolecular assemblies. These structures may be difficult to interpret from a single image because their spatial relationships extend across three dimensions. A reconstructed volume allows researchers to examine how components are arranged within the specimen rather than viewing each feature only as a two-dimensional projection.
Tomography Tilt Series are useful when researchers need three-dimensional and quantitative information about biological specimens. The method connects high-resolution imaging with volumetric analysis, supporting investigations of organization and spatial relationships within cells or other structures. It is especially relevant when a single image cannot adequately convey the architecture or arrangement of the features being studied.