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JoVE Journal
Biology

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A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography
 

A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography

Article DOI: 10.3791/62190-v
March 15th, 2021

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Summary March 15th, 2021

Here, a protocol describing the sample preparation and data collection steps required in cryo soft X-ray tomography (SXT) to image the ultrastructure of whole cryo-preserved cells at a resolution of 25 nm half pitch, is presented.

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