JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Developmental Biology
/
从诱导多能干细胞生成3D全肺类器官,用于模拟肺发育生物学和疾病
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Developmental Biology
Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease
Please note that all translations are automatically generated.
Click here for the English version.
从诱导多能干细胞生成3D全肺类器官,用于模拟肺发育生物学和疾病
DOI:
10.3791/62456-v
•
09:45 min
•
April 12, 2021
•
Sandra L. Leibel
2,3
,
Rachael N. McVicar
3
,
Alicia M. Winquist
3
,
Evan Y. Snyder
2,3
1
Department of Pediatrics
,
University of California, San Diego School of Medicine
,
2
Sanford Consortium for Regenerative Medicine
,
3
Sanford Burnham Prebys Medical Discovery Institute
Chapters
00:04
Introduction
01:05
Definitive Endoderm Induction from Induced Pluripotent Stem Cells (Day 1-3)
03:13
Anterior Foregut Endoderm (AFE) Induction and Lung Progenitor Cell (LPC) Differentiation
05:00
3D lung Organoid Induction (Day 16 22)
06:49
3D Lung Organoid Branching and Maturation
07:11
Results: Characterization of Endoderm and 3D Whole Lung Organoids
09:04
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
本文描述了诱导多能干细胞与含有近端和远端上皮肺细胞以及间充质的三维全肺类器官的逐步定向分化。
Tags
3D Whole Lung Organoids
Induced Pluripotent Stem Cells
Lung Developmental Biology
Lung Disease Modeling
Cryopreservation
Genetic Manipulation
Definitive Endoderm Induction
ROCK Inhibitor
GFR Basement Membrane Matrix
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
诱导多能干细胞使用成纤维样滑膜生成类风湿关节炎患者的关节分离
从人黑色素瘤肿瘤浸润淋巴细胞诱导多能干细胞的产生
从血细胞使用仙台病毒和离心诱导多能干细胞代
从人体外周血单个核细胞融合,无诱导多能干细胞的生成方法游离型载体
脐带血诱导的多能干细胞的软骨形成颗粒形成
人诱导多能干细胞的前脑型脑 Organoids 标准化和可再生性的生成
部分小叶切除术: 形态学肝再生的外科模型
3D Cocultures 星形胶质细胞与人多潜能干细胞神经元的突触电路建模
基于 rna 的人原代成纤维细胞重组成诱导多能干细胞
从脐带血源性多能干细胞中提取三维皮肤有机细胞
Read Article