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从诱导多能干细胞生成3D全肺类器官，用于模拟肺发育生物学和疾病
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Developmental Biology
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JoVE Journal Developmental Biology
Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease
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从诱导多能干细胞生成3D全肺类器官，用于模拟肺发育生物学和疾病

DOI:
10.3791/62456-v

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09:45 min

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April 12, 2021

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, , ,
1Department of Pediatrics,University of California, San Diego School of Medicine, 2Sanford Consortium for Regenerative Medicine, 3Sanford Burnham Prebys Medical Discovery Institute

Chapters

  • 00:04Introduction
  • 01:05Definitive Endoderm Induction from Induced Pluripotent Stem Cells (Day 1-3)
  • 03:13Anterior Foregut Endoderm (AFE) Induction and Lung Progenitor Cell (LPC) Differentiation
  • 05:003D lung Organoid Induction (Day 16 22)
  • 06:493D Lung Organoid Branching and Maturation
  • 07:11Results: Characterization of Endoderm and 3D Whole Lung Organoids
  • 09:04Conclusion

Summary

Automatic Translation

Automatic Translation

本文描述了诱导多能干细胞与含有近端和远端上皮肺细胞以及间充质的三维全肺类器官的逐步定向分化。

Tags

3D Whole Lung OrganoidsInduced Pluripotent Stem CellsLung Developmental BiologyLung Disease ModelingCryopreservationGenetic ManipulationDefinitive Endoderm InductionROCK InhibitorGFR Basement Membrane Matrix

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