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Generering av 3D Hele lungeorganoider fra induserte pluripotente stamceller for modellering av lungeutviklingsbiologi og sykdom
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease
DOI:

09:45 min

April 12, 2021

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Chapters

  • 00:04Introduction
  • 01:05Definitive Endoderm Induction from Induced Pluripotent Stem Cells (Day 1-3)
  • 03:13Anterior Foregut Endoderm (AFE) Induction and Lung Progenitor Cell (LPC) Differentiation
  • 05:003D lung Organoid Induction (Day 16 22)
  • 06:493D Lung Organoid Branching and Maturation
  • 07:11Results: Characterization of Endoderm and 3D Whole Lung Organoids
  • 09:04Conclusion

Summary

Automatic Translation

Artikkelen beskriver trinnvis rettet differensiering av induserte pluripotente stamceller til tredimensjonale hele lungeorganoider som inneholder både proksimale og distale epiteliale lungeceller sammen med mesenchyme.

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