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通过Ussing Chamber技术评估天然组织中肠道紧密连接屏障和离子渗透性的功能评估
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通过Ussing Chamber技术评估天然组织中肠道紧密连接屏障和离子渗透性的功能评估

Article DOI: 10.3791/62468-v • 06:43 min • May 26th, 2021
May 26th, 2021 •
1, 1, 1
1Laboratory of Physiology, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka

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肠上皮不仅提供营养吸收，而且保护免受有毒物质的侵害。顶端最顶端的上皮细胞间连接，即紧密连接，调节细胞旁溶质和离子通透性。这里描述了使用Ussing室技术制备粘膜片和评估紧密连接的离子选择性的方案。

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生物学，第171期，
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