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Biochemistry

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Nano-Differential Scanning Fluorimetry for Screening in Fragment-based Lead Discovery

Article DOI: 10.3791/62469-v • 06:26 min • May 16th, 2021
May 16th, 2021 •
1, 1, 1, 2, 2, 2, 2, 1, 1
1Oncode Institute and Division of Biochemistry, the Netherlands Cancer Institute, 2Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang Goethe-University

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Summary
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Monitoring changes in the melting temperature of a target protein (i.e., thermal shift assay, TSA) is an efficient method for screening fragment libraries of a few hundred compounds. We present a TSA protocol implementing robotics-assisted nano-Differential Scanning Fluorimetry (nano-DSF) for monitoring intrinsic tryptophan fluorescence and light back-scattering for fragment screening.

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Nano-Differential Scanning Fluorimetry Screening Fragment-based Lead Discovery Heat Candidates Protein Targets Ligands Fragment Plate Protein Stock Solution MRC 2-well Crystallization Plate Nano Dispenser Obstacle-free Setup Tab Deck Configuration Plate Definition
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