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JoVE Journal
Biochemistry

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Nano-Differential Scanning Fluorimetry for Screening in Fragment-based Lead Discovery
 

Nano-Differential Scanning Fluorimetry for Screening in Fragment-based Lead Discovery

Article DOI: 10.3791/62469-v
May 16th, 2021

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Summary May 16th, 2021

Monitoring changes in the melting temperature of a target protein (i.e., thermal shift assay, TSA) is an efficient method for screening fragment libraries of a few hundred compounds. We present a TSA protocol implementing robotics-assisted nano-Differential Scanning Fluorimetry (nano-DSF) for monitoring intrinsic tryptophan fluorescence and light back-scattering for fragment screening.

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