Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Detection of SARS-CoV-2 Receptor-Binding Domain…
JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content.

JoVE Journal Immunology and Infection
Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter
Development of an Economical DNA Delivery System by
Journal (Immunology and Infection)
Development of an Economical DNA Delivery System by "Acufection" and its Application to Skin Research
Large-Scale Purification of Porcine or Bovine Photoreceptor Outer Segments for Phagocytosis Assays on Retinal Pigment Epithelial Cells
Journal (Immunology and Infection)
Large-Scale Purification of Porcine or Bovine Photoreceptor Outer Segments for Phagocytosis Assays on Retinal Pigment Epithelial Cells
 

Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter

Article DOI: 10.3791/62488-v • 07:44 min • August 12th, 2021
August 12th, 2021 •
1,2, 1,2, 1,2, 1,2, 3, 1,2, 1,2, 1,2
1Ottawa Hospital Research Institute, 2Department of Biochemistry, Microbiology and Immunology, University of Ottawa, 3Department of Biotechnology, University of Tehran

Chapters

Summary
Automatic Translation

The outlined protocol describes the procedure for producing the HiBiT-receptor-binding domain protein complex and its application for fast and sensitive detection of SARS-CoV-2 antibodies.

Tags

SARS-CoV-2 Receptor-Binding Domain Antibody HiBiT-Based Bioreporter COVID-19 Research Assay Anti-SARS-CoV-2 Antibodies Patient Serum Samples Serological Assays Sensitivity Response Time Cell Culture Dulbecco's Modified Eagle Medium Fetal Bovine Serum Penicillin Streptomycin Biological Safety Cabinet 70% Ethanol Cell Line Petri Dish Confluency Level
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter