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Leukodepletion Filtre-Avledede CD34+ celler som en cellekilde for å studere Megakaryocyte differensiering og blodplatedannelse
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JoVE Journal Biology
Leukodepletion Filters-Derived CD34+ Cells As a Cell Source to Study Megakaryocyte Differentiation and Platelet Formation

Leukodepletion Filtre-Avledede CD34+ celler som en cellekilde for å studere Megakaryocyte differensiering og blodplatedannelse

DOI:
10.3791/62499-v

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06:57 min

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May 20, 2021

•

, , , , ,
1BPPS UMR-S 1255, FMTS,Université de Strasbourg, INSERM, EFS Grand Est

Chapters

  • 00:04Introduction
  • 01:01Leukoreduction Filter (LRF) Back-Flushing Modality Preparation
  • 01:48PBMC Collection
  • 02:47CD34+ Cell Selection
  • 04:01Evaluation of CD34+ Cell Purity
  • 04:35CD34+ Cell Freezing
  • 05:07Results: Mature Megakaryocyte Differentiation and Platelet Formation
  • 06:16Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokollen beskriver i detalj alle trinnene som er involvert i å skaffe leukofilter-avledede CD34+ hematopoietiske forfedre og deres in vitro differensiering og modning i proplateletbærende megakaryocytter som er i stand til å frigjøre blodplater i kulturmediet. Denne prosedyren er nyttig for grundig analyse av cellulære og molekylære mekanismer som kontrollerer megakaryopoiesis.

Tags

Leukodepletion FiltersCD34+ CellsMegakaryocyte DifferentiationPlatelet FormationHematopoietic ProgenitorsMegakaryopoiesisPBMC CollectionDensity Gradient CentrifugationCD34+ Cell SelectionFC Receptor BlockingCD34 Microbeads

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