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Proplatelet Formation Dynamics of Mouse Fresh Bone Marrow Explants
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Proplatelet Formation Dynamics of Mouse Fresh Bone Marrow Explants

Proplatelet Formation Dynamics of Mouse Fresh Bone Marrow Explants

DOI:
10.3791/62501-v

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05:58 min

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May 20, 2021

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, , , ,
1Université de Strasbourg, INSERM, EFS Grand Est, BPPS UMR-S 1255, FMTS

Chapters

  • 00:04Introduction
  • 01:22Preparation of the Experimental Set Up
  • 01:51Bone Marrow Sectioning and Placement into the Incubation Chamber
  • 03:13Real-Time Observation of Marrow Explants and Quantification of the Proplatelet-Extending Megakaryocytes
  • 04:02Results: Morphological Assessment of Megakaryocytes in Explants
  • 04:45Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we detail the bone marrow explant method, from sample preparation to microscopic slide analysis, to evaluate the ability of megakaryocytes which have differentiated in their physiological environment to form proplatelets.

Tags

Keywords: Proplatelet FormationMouse Bone Marrow ExplantsMegakaryocytesReal-time AnalysisThrombophilic DiseaseTyrode’s BufferIncubation ChamberTime-lapse Microscopy

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