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Perle loading proteiner og nukleinsyrer i klæbende menneskelige celler
JoVE Journal
Biology
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JoVE Journal Biology
Bead Loading Proteins and Nucleic Acids into Adherent Human Cells

Perle loading proteiner og nukleinsyrer i klæbende menneskelige celler

DOI:
10.3791/62559-v

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07:28 min

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June 01, 2021

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, , , ,
1Department of Biochemistry and Molecular Biology,Colorado State University, 2World Research Hub Initiative, Institute of Innovative Research,Tokyo Institute of Technology

Chapters

  • 00:04Introduction
  • 01:00Clean, Sterilize, and Dry Glass Beads
  • 02:26Assemble the Bead Loader Apparatus
  • 03:18Bead Loading Cells and Imaging the Bead-Loaded Cells
  • 04:43Results: Proteins and Nucleic Acids Bead-loaded onto Adherent Human Cells
  • 06:49Conclusion

Summary

Automatic Translation

Automatic Translation

Perlebelastning introducerer proteiner, plasmider og partikler i klæbende pattedyrceller. Denne cellebelastningsteknik er billig, hurtig og påvirker ikke cellesundheden væsentligt. Det er bedst egnet til live-celle billeddannelse.

Tags

Bead LoadingAdherent CellsSingle Cell MicroscopyProteinsNucleic AcidsCell LoadingSodium HydroxideEthanolBiosafetyPolypropylene MeshUV SterilizationDesiccant

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