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Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy
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Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy

DOI:
10.3791/62576-v

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14:04 min

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April 25, 2021

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, ,
1Laboratory for Molecular Cell Dynamics, Faculty of Advanced Life Science,Hokkaido University

Chapters

  • 00:00Introduction
  • 01:10Sample Preparation #1- Cell Culture & Transfection
  • 02:51Sample Preparation #2- Cell Lysis & Medium Exchange
  • 04:28FCS Calibration
  • 08:48FCS Measurement
  • 11:45Representative Results
  • 13:03Conclusion

Summary

Automatic Translation

Automatic Translation

We here introduce a procedure to measure protein oligomers and aggregation in cell lysate and live cells using fluorescence correlation spectroscopy.

Tags

Keywords: Protein AggregationFluorescence Correlation SpectroscopyFCSNeurodegenerative DisordersMolecular InteractionsDiffusion PropertiesAmyotrophic Lateral SclerosisNeuro2a CellsCell LysisTDP25GFP Expression

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