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Biology
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用于分析 念珠菌 Cdr1-mGFPHis分析的小尺度等离子膜制备
 
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用于分析 念珠菌 Cdr1-mGFPHis分析的小尺度等离子膜制备

Article DOI: 10.3791/62592-v 09:44 min June 13th, 2021
June 13th, 2021

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本文提出了一个小规模的等离子膜隔离协议,用于描述在糖浆中过度表达的念珠菌ABC(ATP结合盒式)蛋白质Cdr1。 蛋白酶可切割的 C 端 mGFPHis 双标签,Cdr1 和标签之间有 16 残留链接器,旨在促进 Cdr1 的净化和洗涤剂筛选。

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生物学,第172期,
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