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Neuroscience
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Dissection of Single Skeletal Muscle Fibers for Immunofluorescent and Morphometric Analyses of Whole-Mount Neuromuscular Junctions
 

Dissection of Single Skeletal Muscle Fibers for Immunofluorescent and Morphometric Analyses of Whole-Mount Neuromuscular Junctions

Article DOI: 10.3791/62620-v 08:41 min August 14th, 2021
August 14th, 2021

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Summary

The ability to accurately detect neuromuscular junction components is crucial in evaluating modifications in its architecture because of pathological or developmental processes. Here we present a complete description of a straightforward method to obtain high-quality images of whole-mount neuromuscular junctions that can be used to perform quantitative measurements.

Tags

Keywords: Skeletal Muscle Fibers Immunofluorescent Morphometric Analyses Whole-mount Neuromuscular Junctions EDL Muscle Soleus Muscle Dissection Surgical Instruments Fixation Tissue Preparation
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