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JoVE Journal
Neuroscience
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Dissection of Single Skeletal Muscle Fibers for Immunofluorescent and Morphometric Analyses of Whole-Mount Neuromuscular Junctions
 

Dissection of Single Skeletal Muscle Fibers for Immunofluorescent and Morphometric Analyses of Whole-Mount Neuromuscular Junctions

Article DOI: 10.3791/62620-v 08:41 min August 14th, 2021
August 14th, 2021

Chapters

Summary

The ability to accurately detect neuromuscular junction components is crucial in evaluating modifications in its architecture because of pathological or developmental processes. Here we present a complete description of a straightforward method to obtain high-quality images of whole-mount neuromuscular junctions that can be used to perform quantitative measurements.

Tags

Skeletal Muscle Fibers Immunofluorescent Analysis Morphometric Analysis Whole-mount Neuromuscular Junctions Dissection Protocol EDL Muscle Soleus Muscle Surgical Instruments Incision Foot Tendons Annular Ligament Uniband Scissors EDL Tendon Muscle Separation
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