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Immunology and Infection
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Visualization of Gut Microbiota-host Interactions via Fluorescence In Situ Hybridization, Lectin Staining, and Imaging
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Visualization of Gut Microbiota-host Interactions via Fluorescence In Situ Hybridization, Lectin Staining, and Imaging

Visualization of Gut Microbiota-host Interactions via Fluorescence In Situ Hybridization, Lectin Staining, and Imaging

DOI:
10.3791/62646-v

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09:31 min

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July 09, 2021

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,
1School of Biomedical Engineering,University of British Columbia, 2Department of Microbiology and Immunology,University of British Columbia, 3Humans and the Microbiome Program,Canadian Institute for Advanced Research (CIFAR)

Chapters

  • 00:04Introduction
  • 00:56Tissue Sample Collection and Fixation
  • 02:04Paraffin Infiltration and Embedding
  • 02:59Preparation of Slides for Staining and Host Features
  • 04:31Bacterial Staining with FISH
  • 06:41Results: Analysis of the Localization of Muribaculum intestinale Relative to Mucus in a Gnotobiotic Mouse Model and Some Common Imaging Problems
  • 08:33Conclusion

Summary

Automatic Translation

Automatic Translation

This streamlined protocol details a workflow to detect and image bacteria in complex tissue samples, from fixing the tissue to staining microbes with fluorescent in situ hybridization.

Tags

Keywords: Gut MicrobiotaHost-associated MicrobesFluorescence In Situ Hybridization (FISH)Lectin StainingImagingHost-microbiome InterfaceMethacarn FixationParaffin EmbeddingIntestinal SectionsVisualization

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