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Immunology and Infection

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Visualization of Gut Microbiota-host Interactions via Fluorescence In Situ Hybridization, Lectin Staining, and Imaging

Article DOI: 10.3791/62646-v • 09:31 min • July 9th, 2021
July 9th, 2021 •
1,2, 1,2,3
1School of Biomedical Engineering, University of British Columbia, 2Department of Microbiology and Immunology, University of British Columbia, 3Humans and the Microbiome Program, Canadian Institute for Advanced Research (CIFAR)

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Summary
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This streamlined protocol details a workflow to detect and image bacteria in complex tissue samples, from fixing the tissue to staining microbes with fluorescent in situ hybridization.

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Visualization Gut Microbiota-host Interactions Fluorescence In Situ Hybridization Lectin Staining Imaging Host-associated Bacteria Staining Process Visualization Of Microbiome Interface Localization Individual Taxa Bacteria Penetration Host Tissue Host Mucus Depletion Methacarn Solution Intestinal Sections Histology Cassettes
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