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Chapters
- 00:05Title
- 00:56Construction of a Fluorescent Bacterial Strain via Conjugation
- 02:22Production of Axenic Nematode Eggs
- 04:45Co-cultivation Assay with Fluorescent Bacteria
- 05:52Collection and Screening of Nematodes for Bacterial Association by Microscopy
- 06:42Results: Representative Nematode and Bacteria Imagery
- 08:33Conclusion
To study the mutualism between Xenorhabdus bacteria and Steinernema nematodes, methods were developed to monitor bacterial presence and location within nematodes. The experimental approach, which can be applied to other systems, entails engineering bacteria to express the green fluorescent protein and visualizing, using fluorescence microscopy bacteria within the transparent nematode.
Tags
VisualizingBacteriaNematodesFluorescent MicroscopySymbiotic AssociationsXenorhabdus BacteriaSteinernema NematodesModel SystemEntomopathogenicBacterial SymbiontIntestineInfective Juvenile StageNematode SpeciesInteractionsVisualization MethodOptical TransparencyMicroscopyFluorescent ProteinPlasmidsEscherichia Coli Strain
