Name: Closed Chest Biventricular Pressure-Volume Loop Recordings with Admittance Catheters in a Porcine Model (Video) | JoVE Journal
Uploaded: 2021-05-18T15:00:00.000+00:00
Duration: 7 min 56 s
Description: Learn closed chest biventricular pressure-volume loop recordings using admittance catheters in a porcine model. This minimally invasive method enables load-independent hemodynamic evaluation while preserving thoracic physiology.

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This work was supported by the Laerdal Foundation for Acute Medicine (3374), Holger and Ruth Hesse&#39;s Memorial Foundation, S&#248;ster and Verner Lippert&#39;s Foundation, Novo Nordisk Foundation (NNF16OC0023244, NFF17CO0024868), and Alfred Benzon&#39;s Foundation.

<ol><li>Burkhoff, D., Mirsky, I., Suga, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Assessment+of+systolic+and+diastolic+ventricular+properties+via+pressure-volume+analysis%3A+a+guide+for+clinical%2C+translational%2C+and+basic+researchers%5BTitle%5D)+AND+%22American+Journal+of+Physiology-Heart+and+Circulatory+Physiology%22%5BJournal%5D)">Assessment of systolic and diastolic ventricular properties via pressure-volume analysis: a guide for clinical, translational, and basic researchers.</a> American Journal of Physiology-Heart and Circulatory Physiology. 289 (2), 501-512 (2005).</li>
<li>Sagawa, K., Suga, H., Shoukas, A. A., Bakalar, K. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((End-systolic+pressure%2Fvolume+ratio%3A+A+new+index+of+ventricular+contractility%5BTitle%5D)+AND+%22American+Journal+of+Cardiology%22%5BJournal%5D)">End-systolic pressure/volume ratio: A new index of ventricular contractility.</a> American Journal of Cardiology. 40 (5), 748-753 (1977).</li>
<li>Chantler, P. D., Lakatta, E. G., Najjar, S. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Arterial-ventricular+coupling%3A+mechanistic+insights+into+cardiovascular+performance+at+rest+and+during+exercise%5BTitle%5D)+AND+%22Journal+of+Applied+Physiology%22%5BJournal%5D)">Arterial-ventricular coupling: mechanistic insights into cardiovascular performance at rest and during exercise.</a> Journal of Applied Physiology. 105 (4), 1342-1351 (2008).</li>
<li>Axell, R. G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Ventriculo-arterial+coupling+detects+occult+RV+dysfunction+in+chronic+thromboembolic+pulmonary+vascular+disease%5BTitle%5D)+AND+%22Physiological+Reports%22%5BJournal%5D)">Ventriculo-arterial coupling detects occult RV dysfunction in chronic thromboembolic pulmonary vascular disease.</a> Physiological Reports. 5 (7), 13227(2017).</li>
<li>Houser, S. R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Animal+models+of+heart+failure%5BTitle%5D)+AND+%22Circulation+Research%22%5BJournal%5D)">Animal models of heart failure.</a> Circulation Research. 111 (1), 131-150 (2012).</li>
<li>Lahm, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Assessment+of+right+ventricular+function+in+the+research+setting%3A+knowledge+gaps+and+pathways+forward.+An+official+american+thoracic+society+research+statement%5BTitle%5D)+AND+%22American+Journal+of+Respiratory+and+Critical+Care+Medicine%22%5BJournal%5D)">Assessment of right ventricular function in the research setting: knowledge gaps and pathways forward. An official american thoracic society research statement.</a> American Journal of Respiratory and Critical Care Medicine. 198 (4), e15-e43 (2018).</li>
<li>Morimont, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Effective+arterial+elastance+as+an+index+of+pulmonary+vascular+load%5BTitle%5D)+AND+%22American+Journal+of+Physiology+-+Heart+and+Circulatory+Physiology%22%5BJournal%5D)">Effective arterial elastance as an index of pulmonary vascular load.</a> American Journal of Physiology - Heart and Circulatory Physiology. 294 (6), 2736-2742 (2008).</li>
<li>Kutty, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Validation+of+admittance+computed+left+ventricular+volumes+against+real-time+three-dimensional+echocardiography+in+the+porcine+heart%5BTitle%5D)+AND+%22Experimental+Physiology%22%5BJournal%5D)">Validation of admittance computed left ventricular volumes against real-time three-dimensional echocardiography in the porcine heart.</a> Experimental Physiology. 98 (6), 1092-1101 (2013).</li>
<li>Bove, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Acute+and+chronic+effects+of+dysfunction+of+right+ventricular+outflow+tract+components+on+right+ventricular+performance+in+a+porcine+model%3A+Implications+for+primary+repair+of+tetralogy+of+fallot%5BTitle%5D)+AND+%22Journal+of+the+American+College+of+Cardiology%22%5BJournal%5D)">Acute and chronic effects of dysfunction of right ventricular outflow tract components on right ventricular performance in a porcine model: Implications for primary repair of tetralogy of fallot.</a> Journal of the American College of Cardiology. 60 (1), 64-71 (2012).</li>
<li>Townsend, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Measuring+pressure+volume+loops+in+the+mouse%5BTitle%5D)+AND+%22Journal+of+Visualized+Experiments%22%5BJournal%5D)">Measuring pressure volume loops in the mouse.</a> Journal of Visualized Experiments. (111), e53810(2016).</li>
<li>Belenkie, I., Smith, E. R., Tyberg, J. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Ventricular+interaction%3A+From+bench+to+bedside%5BTitle%5D)+AND+%22Annals+of+Medicine%22%5BJournal%5D)">Ventricular interaction: From bench to bedside.</a> Annals of Medicine. 33 (4), 236-241 (2009).</li>
<li>LaCorte, J. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Correlation+of+the+TIE+index+with+invasive+measurements+of+ventricular+function+in+a+porcine+model%5BTitle%5D)+AND+%22Journal+of+the+American+Society+of+Echocardiography%22%5BJournal%5D)">Correlation of the TIE index with invasive measurements of ventricular function in a porcine model.</a> Journal of the American Society of Echocardiography. 16 (5), 442-447 (2003).</li>
<li>Amà, R., Leather, H. A., Segers, P., Vandermeersch, E., Wouters, P. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Acute+pulmonary+hypertension+causes+depression+of+left+ventricular+contractility+and+relaxation%5BTitle%5D)+AND+%22European+Journal+of+Anaesthesiology%22%5BJournal%5D)">Acute pulmonary hypertension causes depression of left ventricular contractility and relaxation.</a> European Journal of Anaesthesiology. 23 (10), 824-831 (2006).</li>
<li>Missant, C., Rex, S., Segers, P., Wouters, P. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Levosimendan+improves+right+ventriculovascular+coupling+in+a+porcine+model+of+right+ventricular+dysfunction%5BTitle%5D)+AND+%22Critical+Care+Medicine%22%5BJournal%5D)">Levosimendan improves right ventriculovascular coupling in a porcine model of right ventricular dysfunction.</a> Critical Care Medicine. 35 (3), 707-715 (2007).</li>
<li>Mortensen, C. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Impact+of+preload+on+right+ventricular+hemodynamics+in+acute+pulmonary+embolism%5BTitle%5D)+AND+%22Critical+Care+Medicine%22%5BJournal%5D)">Impact of preload on right ventricular hemodynamics in acute pulmonary embolism.</a> Critical Care Medicine. 48 (12), 1306-1312 (2020).</li>
<li>Kramer, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Inhaled+nitric+oxide+has+pulmonary+vasodilator+efficacy+both+in+the+immediate+and+prolonged+phase+of+acute+pulmonary+embolism%5BTitle%5D)+AND+%22European+Heart+Journal%3A+Acute+Cardiovascular+Care%22%5BJournal%5D)">Inhaled nitric oxide has pulmonary vasodilator efficacy both in the immediate and prolonged phase of acute pulmonary embolism.</a> European Heart Journal: Acute Cardiovascular Care. , 204887262091871(2020).</li>
<li>Lyhne, M. D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Oxygen+therapy+lowers+right+ventricular+afterload+in+experimental+acute+pulmonary+embolism%5BTitle%5D)+AND+%22Critical+Care+Medicine%22%5BJournal%5D)">Oxygen therapy lowers right ventricular afterload in experimental acute pulmonary embolism.</a> Critical Care Medicine. , (2021).</li>
<li>Lyhne, M. D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Right+ventricular+adaptation+in+the+critical+phase+after+acute+intermediate-risk+pulmonary+embolism%5BTitle%5D)+AND+%22European+Heart+Journal%3A+Acute+Cardiovascular+Care%22%5BJournal%5D)">Right ventricular adaptation in the critical phase after acute intermediate-risk pulmonary embolism.</a> European Heart Journal: Acute Cardiovascular Care. , 204887262092525(2020).</li>
<li>Dietrichs, E. S., Tveita, T., Smith, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Hypothermia+and+cardiac+electrophysiology%3A+a+systematic+review+of+clinical+and+experimental+data%5BTitle%5D)+AND+%22Cardiovascular+Research%22%5BJournal%5D)">Hypothermia and cardiac electrophysiology: a systematic review of clinical and experimental data.</a> Cardiovascular Research. 115 (3), 501-509 (2018).</li>
<li>Boulate, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Early+development+of+right+ventricular+ischemic+lesions+in+a+novel+large+animal+model+of+acute+right+heart+failure+in+chronic+thromboembolic+pulmonary+hypertension%5BTitle%5D)+AND+%22Journal+of+Cardiac+Failure%22%5BJournal%5D)">Early development of right ventricular ischemic lesions in a novel large animal model of acute right heart failure in chronic thromboembolic pulmonary hypertension.</a> Journal of Cardiac Failure. 23 (12), 876-886 (2017).</li>
<li>Haney, M. F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Myocardial+systolic+function+increases+during+positive+pressure+lung+inflation%5BTitle%5D)+AND+%22Anesthesia+and+Analgesia%22%5BJournal%5D)">Myocardial systolic function increases during positive pressure lung inflation.</a> Anesthesia and Analgesia. 101 (5), 1269-1274 (2005).</li>
<li>Gorcsan, J., Strum, D. P., Mandarino, W. A., Gulati, V. K., Pinsky, M. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Quantitative+assessment+of+alterations+in+regional+left+ventricular+contractility+with+color-coded+tissue+doppler+echocardiography%3A+Comparison+with+sonomicrometry+and+pressure-volume+relations%5BTitle%5D)+AND+%22Circulation%22%5BJournal%5D)">Quantitative assessment of alterations in regional left ventricular contractility with color-coded tissue doppler echocardiography: Comparison with sonomicrometry and pressure-volume relations.</a> Circulation. 95 (10), 2423-2433 (1997).</li>
<li>Pinsky, M. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Dynamic+right+and+left+ventricular+interactions+in+the+pig%5BTitle%5D)+AND+%22Experimental+Physiology%22%5BJournal%5D)">Dynamic right and left ventricular interactions in the pig.</a> Experimental Physiology. 105 (8), 1293-1315 (2020).</li>
<li>Mitchell, J. R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((RV+filling+modulates+LV+function+by+direct+ventricular+interaction+during+mechanical+ventilation%5BTitle%5D)+AND+%22American+Journal+of+Physiology-Heart+and+Circulatory+Physiology%22%5BJournal%5D)">RV filling modulates LV function by direct ventricular interaction during mechanical ventilation.</a> American Journal of Physiology-Heart and Circulatory Physiology. 289 (2), 549-557 (2005).</li>
<li>Larson, E. R., Feldman, M. D., Valvano, J. W., Pearce, J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Analysis+of+the+spatial+sensitivity+of+conductance%2Fadmittance+catheter+ventricular+volume+estimation%5BTitle%5D)+AND+%22IEEE+Transactions+on+Biomedical+Engineering%22%5BJournal%5D)">Analysis of the spatial sensitivity of conductance/admittance catheter ventricular volume estimation.</a> IEEE Transactions on Biomedical Engineering. 60 (8), 2316-2324 (2013).</li>
<li>Hout, G. P. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Admittance-based+pressure-volume+loops+versus+gold+standard+cardiac+magnetic+resonance+imaging+in+a+porcine+model+of+myocardial+infarction%5BTitle%5D)+AND+%22Physiological+Reports%22%5BJournal%5D)">Admittance-based pressure-volume loops versus gold standard cardiac magnetic resonance imaging in a porcine model of myocardial infarction.</a> Physiological Reports. 2 (4), 00287(2014).</li>
<li>Baker, A. E., Dani, R., Smith, E. R., Tyberg, J. V., Belenkie, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Quantitative+assessment+of+independent+contributions+of+pericardium+and+septum+to+direct+ventricular+interaction%5BTitle%5D)+AND+%22American+Journal+of+Physiology-Heart+and+Circulatory+Physiology%22%5BJournal%5D)">Quantitative assessment of independent contributions of pericardium and septum to direct ventricular interaction.</a> American Journal of Physiology-Heart and Circulatory Physiology. 275 (2), 476-483 (1998).</li>
<li>Sanz, J., Sánchez-Quintana, D., Bossone, E., Bogaard, H. J., Naeije, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Anatomy%2C+function%2C+and+dysfunction+of+the+right+ventricle%5BTitle%5D)+AND+%22Journal+of+the+American+College+of+Cardiology%22%5BJournal%5D)">Anatomy, function, and dysfunction of the right ventricle.</a> Journal of the American College of Cardiology. 73 (12), 1463-1482 (2019).</li>
<li>Gavazzoni, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Prognostic+value+of+right+ventricular+free+wall+longitudinal+strain+in+a+large+cohort+of+outpatients+with+left-side+heart+disease%5BTitle%5D)+AND+%22European+Heart+Journal%3A+Cardiovascular+Imaging%22%5BJournal%5D)">Prognostic value of right ventricular free wall longitudinal strain in a large cohort of outpatients with left-side heart disease.</a> European Heart Journal: Cardiovascular Imaging. 21 (9), 1013-1021 (2019).</li>
<li>Berglund, F., Piña, P., Herrera, C. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Right+ventricle+in+heart+failure+with+preserved+ejection+fraction%5BTitle%5D)+AND+%22Heart%22%5BJournal%5D)">Right ventricle in heart failure with preserved ejection fraction.</a> Heart. 106 (23), 1798-1804 (2020).</li>
</ol>

None of the authors has any conflicts of interest to declare.

This paper describes a reproducible minimally invasive closed chest approach for bi-ventricular pressure-volume loop recordings.
Advancement of the PV catheter from the RA into the RV is the most critical step in this protocol. The complex composition of the RV and the stiffness of the catheter complicate insertion into the easily distended and geometrically challenging RV. This difficulty may explain why open chest instrumentation is often preferred. During pilot studies, numerous accesses an...

Pressure-volume (PV) loops contain a large number of hemodynamic information, including end-systolic and end-diastolic pressures and volumes, ejection fraction, stroke volume, and stroke work1. Furthermore, transient preload reduction creates a family of loops from which load-independent variables can be derived2,3. This load-independent evaluation of ventricular function makes PV loop recordings state-of-the-art in hemodynamic evaluation. PV loop recording can be performed in humans but is primarily used and recommended in preclinical research4,5,6.
Pressure-volume loops can be obtained from both the right ventricle (RV) and the left ventricle (LV). Most research hypotheses are focused on a single ventricle, resulting in only univentricular PV loops being recorded7,8,9,10. However, the right and left ventricles exert systolic and diastolic interdependence due to their serial and parallel connections within the tight pericardium11. Changes in the output or the size of one ventricle will affect the size, loading conditions, or perfusion of the other ventricle. Thus, bi-ventricular PV loop recordings provide a more comprehensive evaluation of the total cardiac performance. Pharmacological interventions may also affect the two ventricles and their loading conditions differently, further emphasizing the importance of bi-ventricular evaluation.
PV catheters can be advanced into either ventricle by several approaches, including open chest approach with access from the apex of the heart or through the RV outflow tract7,10,12,13,14. However, the opening of the thorax will affect the physiological conditions and may introduce bias.
Based on our experience from previous studies15,16,17,18, we aim to present our closed chest approach to bi-ventricular PV loop recordings in a large animal model of acute RV failure having minimal influence on cardiopulmonary physiology (Figure 1).

<table><tbody><tr><td>12L-RS</td><td>GE Healthcare Japan</td><td>5141337</td><td>Ultrasound probe</td></tr><tr><td>12L-RS</td><td>GE Healthcare Japan</td><td>5141337</td><td>Ultrasound probe</td></tr><tr><td>Adhesive Aperature Drape (OneMed)</td><td>evercare</td><td>1515-01</td><td>75 x 90 cm (hole: 6 x 8 cm)</td></tr><tr><td>Adhesive Aperature Drape (OneMed)</td><td>evercare</td><td>1515-01</td><td>75 x 90 cm (hole: 6 x 8 cm)</td></tr><tr><td>Alaris GP Guardrails plus</td><td>CareFusion</td><td>9002TIG01-G</td><td>Infusion pump</td></tr><tr><td>Alaris GP Guardrails plus</td><td>CareFusion</td><td>9002TIG01-G</td><td>Infusion pump</td></tr><tr><td>Alaris Infusion set</td><td>BD Plastipak</td><td>60593</td><td></td></tr><tr><td>Alaris Infusion set</td><td>BD Plastipak</td><td>60593</td><td></td></tr><tr><td>Alkoholswap</td><td>MEDIQ Danmark</td><td>3340012</td><td>82% ethanol, 0,5% chlorhexidin, skin disinfection</td></tr><tr><td>Alkoholswap</td><td>MEDIQ Danmark</td><td>3340012</td><td>82% ethanol, 0,5% chlorhexidin, skin disinfection</td></tr><tr><td>Amplatz Support Wire Guide Extra-Stiff</td><td>Cook Medical</td><td>THSF-25-260-AES</td><td>diameter: 0.025 inches, length: 260 cm</td></tr><tr><td>Amplatz Support Wire Guide Extra-Stiff</td><td>Cook Medical</td><td>THSF-25-260-AES</td><td>diameter: 0.025 inches, length: 260 cm</td></tr><tr><td>BD Connecta</td><td>BD</td><td>394601</td><td>Luer-Lock</td></tr><tr><td>BD Connecta</td><td>BD</td><td>394601</td><td>Luer-Lock</td></tr><tr><td>BD Emerald</td><td>BD</td><td>307736</td><td>10 mL syringe</td></tr><tr><td>BD Emerald</td><td>BD</td><td>307736</td><td>10 mL syringe</td></tr><tr><td>BD Luer-Lock</td><td>BD Plastipak</td><td>300865</td><td>BD = Becton Dickinson, 50 mL syringe</td></tr><tr><td>BD Luer-Lock</td><td>BD Plastipak</td><td>300865</td><td>BD = Becton Dickinson, 50 mL syringe</td></tr><tr><td>BD Platipak</td><td>BD</td><td>300613</td><td>20 mL syringe</td></tr><tr><td>BD Platipak</td><td>BD</td><td>300613</td><td>20 mL syringe</td></tr><tr><td>BD Venflon Pro</td><td>Becton Dickinson Infusion Therapy</td><td>393204</td><td>20G</td></tr><tr><td>BD Venflon Pro</td><td>Becton Dickinson Infusion Therapy</td><td>393204</td><td>20G</td></tr><tr><td>BD Venflon Pro</td><td>Becton Dickinson Infusion Therapy</td><td>393208</td><td>17G</td></tr><tr><td>BD Venflon Pro</td><td>Becton Dickinson Infusion Therapy</td><td>393208</td><td>17G</td></tr><tr><td>Butomidor Vet</td><td>Richter Pharma AG</td><td>531943</td><td>10 mg/mL</td></tr><tr><td>Butomidor Vet</td><td>Richter Pharma AG</td><td>531943</td><td>10 mg/mL</td></tr><tr><td>Check-Flo Performer Introducer</td><td>Cook Medical</td><td>RCFW-16.0P-38-30-RB</td><td>16 F sheath, 30 cm long</td></tr><tr><td>Check-Flo Performer Introducer</td><td>Cook Medical</td><td>RCFW-16.0P-38-30-RB</td><td>16 F sheath, 30 cm long</td></tr><tr><td>Cios Connect S/N 20015</td><td>Siemens Healthineers</td><td></td><td>C-arm</td></tr><tr><td>Cios Connect S/N 20015</td><td>Siemens Healthineers</td><td></td><td>C-arm</td></tr><tr><td>D-LCC12A-01</td><td>GE Healthcare Finland</td><td></td><td>Pressure measurement monitor</td></tr><tr><td>D-LCC12A-01</td><td>GE Healthcare Finland</td><td></td><td>Pressure measurement monitor</td></tr><tr><td>Durapore</td><td>3M</td><td>-</td><td>Adhesive tape</td></tr><tr><td>Durapore</td><td>3M</td><td>-</td><td>Adhesive tape</td></tr><tr><td>E-PRESTIN-00</td><td>GE Healthcare Finland</td><td>6152932</td><td>Respirator tubes</td></tr><tr><td>E-PRESTIN-00</td><td>GE Healthcare Finland</td><td>6152932</td><td>Respirator tubes</td></tr><tr><td>Exagon vet</td><td>Richter Pharma AG</td><td>427931</td><td>400 mg/mL</td></tr><tr><td>Exagon vet</td><td>Richter Pharma AG</td><td>427931</td><td>400 mg/mL</td></tr><tr><td>Fast-Cath Hemostasis Introducer 12F</td><td>St. Jude Medical</td><td>406128</td><td>L: 12 cm</td></tr><tr><td>Fast-Cath Hemostasis Introducer 12F</td><td>St. Jude Medical</td><td>406128</td><td>L: 12 cm</td></tr><tr><td>Favorita II</td><td>Aesculap</td><td></td><td>Type: GT104</td></tr><tr><td>Favorita II</td><td>Aesculap</td><td></td><td>Type: GT104</td></tr><tr><td>Fentanyl</td><td>B. Braun</td><td>71036</td><td>50 mikrogram/mL</td></tr><tr><td>Fentanyl</td><td>B. Braun</td><td>71036</td><td>50 mikrogram/mL</td></tr><tr><td>Ketaminol Vet</td><td>MSD/Intervet International B.V.</td><td>511519</td><td>100 mg/mL</td></tr><tr><td>Ketaminol Vet</td><td>MSD/Intervet International B.V.</td><td>511519</td><td>100 mg/mL</td></tr><tr><td>LabChart</td><td>ADInstruments</td><td></td><td>Data aquisition software</td></tr><tr><td>LabChart</td><td>ADInstruments</td><td></td><td>Data aquisition software</td></tr><tr><td>Lawton 85-0010 ZK1</td><td>Lawton</td><td></td><td>Laryngoscope</td></tr><tr><td>Lawton 85-0010 ZK1</td><td>Lawton</td><td></td><td>Laryngoscope</td></tr><tr><td>Lectospiral</td><td>VYGON</td><td>1159.90</td><td>400 cm (Luer-LOCK)</td></tr><tr><td>Lectospiral</td><td>VYGON</td><td>1159.90</td><td>400 cm (Luer-LOCK)</td></tr><tr><td>Lubrithal eye gel</td><td>Dechra, Great Britain</td><td></td><td></td></tr><tr><td>Lubrithal eye gel</td><td>Dechra, Great Britain</td><td></td><td></td></tr><tr><td>MBH qufora</td><td>MBH-International A/S</td><td>13853401</td><td>Urine bag</td></tr><tr><td>MBH qufora</td><td>MBH-International A/S</td><td>13853401</td><td>Urine bag</td></tr><tr><td>Natriumklorid</td><td>Fresenius Kabi</td><td>7340022100528</td><td>9 mg/ml Isotonic saline</td></tr><tr><td>Natriumklorid</td><td>Fresenius Kabi</td><td>7340022100528</td><td>9 mg/ml Isotonic saline</td></tr><tr><td>PICO50 Aterial Blood Sampler</td><td>Radiometer</td><td>956-552</td><td>2 mL</td></tr><tr><td>PICO50 Aterial Blood Sampler</td><td>Radiometer</td><td>956-552</td><td>2 mL</td></tr><tr><td>Portex Tracheal Tube</td><td>Smiths Medical</td><td>100/150/075</td><td>&quot;Cuffed Clear Oral/Nasal Murphy Eye&quot;</td></tr><tr><td>Portex Tracheal Tube</td><td>Smiths Medical</td><td>100/150/075</td><td>&quot;Cuffed Clear Oral/Nasal Murphy Eye&quot;</td></tr><tr><td>PowerLab 16/35</td><td>ADInstruments</td><td>PL3516</td><td>Serial number: 3516-1841</td></tr><tr><td>PowerLab 16/35</td><td>ADInstruments</td><td>PL3516</td><td>Serial number: 3516-1841</td></tr><tr><td>Pressure Extension set</td><td>CODAN</td><td>7,14,020</td><td>Tube for anesthetics, 150 cm long, inner diameter 0.9 mm</td></tr><tr><td>Pressure Extension set</td><td>CODAN</td><td>7,14,020</td><td>Tube for anesthetics, 150 cm long, inner diameter 0.9 mm</td></tr><tr><td>Propolipid</td><td>Fresenius Kabi</td><td>21636</td><td>Propofol, 10 mg/mL</td></tr><tr><td>Propolipid</td><td>Fresenius Kabi</td><td>21636</td><td>Propofol, 10 mg/mL</td></tr><tr><td>PTS-X</td><td>NuMED Canada Inc.</td><td>PTSX253</td><td>Inferior vena cava balloon</td></tr><tr><td>PTS-X</td><td>NuMED Canada Inc.</td><td>PTSX253</td><td>Inferior vena cava balloon</td></tr><tr><td>Radiofocus Introducer II</td><td>Radiofocus/Terumo</td><td>RS+B80N10MQ</td><td>6+7+8F sheaths</td></tr><tr><td>Radiofocus Introducer II</td><td>Radiofocus/Terumo</td><td>RS+B80N10MQ</td><td>6+7+8F sheaths</td></tr><tr><td>Rompun Vet</td><td>Beyer</td><td>86450917</td><td>Xylazin, 20 mg/mL</td></tr><tr><td>Rompun Vet</td><td>Beyer</td><td>86450917</td><td>Xylazin, 20 mg/mL</td></tr><tr><td>R&amp;uuml;sch Brilliant AquaFlate Glycerine</td><td>Teleflex</td><td>178000</td><td>Bladder catheter, size 14</td></tr><tr><td>R&amp;uuml;sch Brilliant AquaFlate Glycerine</td><td>Teleflex</td><td>178000</td><td>Bladder catheter, size 14</td></tr><tr><td>S/5 Avance</td><td>Datex-Ohmeda</td><td>-</td><td>Mechanical ventilator</td></tr><tr><td>S/5 Avance</td><td>Datex-Ohmeda</td><td>-</td><td>Mechanical ventilator</td></tr><tr><td>Safersonic Conti Plus &amp;amp; Safergel</td><td>SECMA medical innovation</td><td>SAF.612.18120.WG.SEC</td><td>18 x 120 cm (Safersonic Sterile Transducer Cover with Adhesive Area and Safergel)</td></tr><tr><td>Safersonic Conti Plus &amp;amp; Safergel</td><td>SECMA medical innovation</td><td>SAF.612.18120.WG.SEC</td><td>18 x 120 cm (Safersonic Sterile Transducer Cover with Adhesive Area and Safergel)</td></tr><tr><td>Scisense Catheter</td><td>Transonic Scisense</td><td>FDH-5018B-E245B</td><td>Serial number: 50-533. Pressure-volume catheter</td></tr><tr><td>Scisense Catheter</td><td>Transonic Scisense</td><td>FDH-5018B-E245B</td><td>Serial number: 50-533. Pressure-volume catheter</td></tr><tr><td>Scisense Pressure-Volume Measurement System</td><td>Transonic Scisense</td><td>ADV500</td><td>Model: FY097B. Pressure-volume box</td></tr><tr><td>Scisense Pressure-Volume Measurement System</td><td>Transonic Scisense</td><td>ADV500</td><td>Model: FY097B. Pressure-volume box</td></tr><tr><td>Swan-Ganz CCOmbo</td><td>Edwards Lifesciences</td><td>744F75</td><td>110 cm</td></tr><tr><td>Swan-Ganz CCOmbo</td><td>Edwards Lifesciences</td><td>744F75</td><td>110 cm</td></tr><tr><td>TruWave Pressure Monitoring Set</td><td>Edwards Lifesciences</td><td>T434303A</td><td>210 cm</td></tr><tr><td>TruWave Pressure Monitoring Set</td><td>Edwards Lifesciences</td><td>T434303A</td><td>210 cm</td></tr><tr><td>Vivid iq</td><td>GE Medical Systems China</td><td>Vivid iq</td><td></td></tr><tr><td>Vivid iq</td><td>GE Medical Systems China</td><td>Vivid iq</td><td></td></tr><tr><td>Zoletil 50 Vet (tiletamin 125 mg and zolazepam 125 mg)</td><td>Virbac</td><td>83046805</td><td>Zoletil Mix for pigs: 1 vial of Zoletil 50 Vet (dry matter); add 6.25 mL Xylozin (20 mg/mL), 1.25 mL ketamin (100 mg/mL) and 2.5 mL Butorphanol (10 mg/mL). Dose for pre-anesthesia: 10 mL/10 kg as intramuscular injection</td></tr><tr><td>Zoletil 50 Vet (tiletamin 125 mg and zolazepam 125 mg)</td><td>Virbac</td><td>83046805</td><td>Zoletil Mix for pigs: 1 vial of Zoletil 50 Vet (dry matter); add 6.25 mL Xylozin (20 mg/mL), 1.25 mL ketamin (100 mg/mL) and 2.5 mL Butorphanol (10 mg/mL). Dose for pre-anesthesia: 10 mL/10 kg as intramuscular injection</td></tr></tbody></table>

closed chest biventricular pressure-volume loop recordings with admittance catheters in a porcine model

Closed Chest Biventricular Pressure-Volume Loop Recordings with Admittance Catheters in a Porcine Model

Pressure-volume (PV) loop recording enables the state-of-the-art investigation of load-independent variables of ventricular performance. Uni-ventricular evaluation is often performed in preclinical research. However, the right and left ventricles exert functional interdependence due to their parallel and serial connections, encouraging simultaneous evaluation of both ventricles. Furthermore, various pharmacological interventions may affect the ventricles and their preloads and afterloads differently. 
We describe our closed chest approach to admittance-based bi-ventricular PV loop recordings in a porcine model of acute right ventricular (RV) overload. We utilize minimally invasive techniques with all vascular accesses guided by ultrasound. PV catheters are positioned, under fluoroscopic guidance, to avoid thoracotomy in animals, as the closed chest approach maintains the relevant cardiopulmonary physiology. The admittance technology provides real-time PV loop recordings without the need for post-hoc processing. Furthermore, we explain some essential troubleshooting steps during critical timepoints of the presented procedure. 
The presented protocol is a reproducible and physiologically relevant approach to obtain a bi-ventricular cardiac PV loop recording in a large animal model. This can be applied to a large variety of cardiovascular animal research.

The present instructions describe an approach to achieve admittance-based PV recordings from both the RV and the LV in a large animal.
To compare our simultaneous PV recordings in the RV and LV, we performed a linear regression of the bi-ventricular CO measurements from our largest study18 with the highest number of simultaneous RV CO and LV CO measurements (n=379 recordings from 12 animals). We found that the slope was 1.03 (95%CI 0.90-1.15) with a Y-intercept of 695 (...

Here we present a closed chest approach to admittance-based bi-ventricular pressure-volume loop recordings in pigs with acute right ventricular dysfunction.

Pressure-volume (PV) loop recording enables the state-of-the-art investigation of load-independent variables of ventricular performance. Uni-ventricular ...

closed-chest-biventricular-pressure-volume-loop-recordings-with

Research

JoVE Journal

Medicine

Most studies involving the Langendorff apparatus use small animal models due to the increased complexity of systems for larger mammals. We describe a Langendorff system for large animal models that allows for use across a range of species, including humans, and relatively easy data acquisition.

An Isolated Working Heart System for Large Animal Models

This article describes the measurement of murine left ventricular function via pressure/volume analysis at different heart rates.

Cardiac Catheterization in Mice to Measure the Pressure Volume Relationship: Investigating the Bowditch Effect

Cardiac pressure-volume loop analysis is the most comprehensive way to measure cardiac function in the intact heart. We describe a technique to perform and analyze cardiac pressure volume loops, using conductance catheters.

Cardiac Pressure-Volume Loop Analysis Using Conductance Catheters in Mice

Chronic Thromboembolic Pulmonary Hypertension (CTEPH) and Right Ventricular (RV) dysfunction were induced in piglets by progressive obstruction of the pulmonary arteries. Consequences were remarkably similar to those observed in CTEPH patients. This animal model would be a very useful tool for pathophysiology and therapeutic experiments on CTEPH and RV failure.

Chronic Thromboembolic Pulmonary Hypertension and Assessment of Right Ventricular Function in the Piglet

Here we present a protocol to assess cardiopulmonary function in awake swine, at rest and during graded treadmill exercise. Chronic instrumentation allows for repeated hemodynamic measurements uninfluenced by cardiodepressive anesthetic agents.

Surgical Placement of Catheters for Long-term Cardiovascular Exercise Testing in Swine

Reliable and accurate outcome assessment is the key for translation of preclinical therapies into clinical treatment. The current paper describes how to assess three clinically relevant primary outcome parameters of cardiac performance and damage in a pig acute myocardial infarction model.

Primary Outcome Assessment in a Pig Model of Acute Myocardial Infarction

We present a protocol to induce and phenotype an acute right heart failure in a large animal model with chronic pulmonary hypertension. This model can be used to test therapeutic interventions, to develop right heart metrics or to improve the understanding of acute right heart failure pathophysiology.

Induction and Phenotyping of Acute Right Heart Failure in a Large Animal Model of Chronic Thromboembolic Pulmonary Hypertension

Presented here is a protocol to assess biventricular heart function in mice by generating pressure-volume (PV) loops from the right and left ventricle in the same animal using closed chest catheterization. The focus is on the technical aspect of surgery and data acquisition.

Biventricular Assessment of Cardiac Function and Pressure-Volume Loops by Closed-Chest Catheterization in Mice

The protocol here shows how continuous administration of crystalloids into the central veins of a euvolemic pig/piglet allows for the appropriate investigation of the physiological effects of acute volume overload.

Adult and Pediatric Porcine Model of Acute Volume Overload

Surgical trauma induces an inflammatory response. Cytokines and endogenous ligands are known to modulate myocardial infarct size following ischemia and reperfusion. We present a modified closed-chest model of murine ischemia and reperfusion using hanging weights to minimize effects of thoracotomy.

A Murine Closed-chest Model of Myocardial Ischemia and Reperfusion

Learn closed chest biventricular pressure-volume loop recordings using admittance catheters in a porcine model. This minimally invasive method enables load-independent hemodynamic evaluation while preserving thoracic physiology.

Closed Chest Biventricular Pressure-Volume Loop Recordings with Admittance Catheters in a Porcine Model

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