Journal
/
Developmental Biology
/
2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development
JoVE Journal
Developmental Biology
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development

2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development

DOI:
10.3791/62667-v

•

14:19 min

•

March 25, 2022

•

, , , , , , , , , , , , , , , ,
1Imagine Institute, INSERM UMR 1163,Université de Paris, 2Imagine Institute, iPSC Core Facility, INSERM UMR U1163,Université de Paris, 3Necker Bio-image Analysis platform of the SFR Necker, 4Imagine Institute, Cell Imaging Platform, INSERM-US24-CNRS UMS 3633 Structure Fédérative de Recherche Necker, INSERM UMR U1163,Université de Paris, 5Fédération de Génétique, Hôpital Necker-Enfants Malades,Assistance Publique Hôpitaux de Paris, 6Pediatric Neurology,APHP- Necker Enfants Malades Hospital

Chapters

  • 00:07Introduction
  • 01:42Generation of 2D hIPS Cell-Based Models of Neocortical Development
  • 02:56Generation of Dorsal Forebrain Organoids
  • 04:56In toto Immunolabeling, Clearing, and Lightsheet Acquisition of Dorsal Forebrain Organoids
  • 07:00Immunostaining and Clearing of Free-Floating Sections of Dorsal Forebrain Organoids
  • 09:20Representative Results
  • 13:11Conclusion

Summary

Automatic Translation

Automatic Translation

We present detailed protocols for the generation and characterization of 2D and 3D human induced pluripotent stem cell (hIPSC)-based models of neocortical development as well as complementary methodologies enabling qualitative and quantitative analysis of primary cilium (PC) biogenesis and function.

Tags

Keywords: 2D3DHumanInduced Pluripotent Stem CellPrimary CiliumNeocortical DevelopmentNeural RosettesDorsal Forebrain OrganoidsEmbryoid BodyDual SMAD InhibitionImmunohistochemistryVibratomeConfocal Microscopy

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/62667?article_type=v

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation
check_url/62667?article_type=v

Three-dimensional Quantification of Dendritic Spines from Pyramidal Neurons Derived from Human Induced Pluripotent Stem Cells
check_url/62667?article_type=v

Cultivate Primary Nasal Epithelial Cells from Children and Reprogram into Induced Pluripotent Stem Cells
check_url/62667?article_type=v

Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells
check_url/62667?article_type=v

Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells
check_url/62667?article_type=v

Synaptic Microcircuit Modeling with 3D Cocultures of Astrocytes and Neurons from Human Pluripotent Stem Cells
check_url/62667?article_type=v

Microfluidic Assay for the Assessment of Leukocyte Adhesion to Human Induced Pluripotent Stem Cell-derived Endothelial Cells (hiPSC-ECs)
check_url/62667?article_type=v

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells
check_url/62667?article_type=v

Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells
check_url/62667?article_type=v

In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells

Read Article