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Journal / Developmental Biology / 2D og 3D Human induserte Pluripotente…
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Developmental Biology
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JoVE Journal Developmental Biology
2D og 3D Human induserte Pluripotente stamcellebaserte modeller for å dissekere primær ciliuminvolvering under neokortisk utvikling
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2D og 3D Human induserte Pluripotente stamcellebaserte modeller for å dissekere primær ciliuminvolvering under neokortisk utvikling

Article DOI: 10.3791/62667-v • 14:19 min • March 25th, 2022
March 25th, 2022 •
*1, *1, 2, 1, 1, 1, 3, 1, 1, 1, 4, 1, 1, 1,5, 1,6, 2, 1
1Imagine Institute, INSERM UMR 1163, Université de Paris, 2Imagine Institute, iPSC Core Facility, INSERM UMR U1163, Université de Paris, 3Necker Bio-image Analysis platform of the SFR Necker, 4Imagine Institute, Cell Imaging Platform, INSERM-US24-CNRS UMS 3633 Structure Fédérative de Recherche Necker, INSERM UMR U1163, Université de Paris, 5Fédération de Génétique, Hôpital Necker-Enfants Malades, Assistance Publique Hôpitaux de Paris, 6Pediatric Neurology, APHP- Necker Enfants Malades Hospital
* These authors contributed equally

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Vi presenterer detaljerte protokoller for generering og karakterisering av 2D og 3D human indusert pluripotent stamcelle (hIPSC)-baserte modeller for neokortisk utvikling samt komplementære metoder som muliggjør kvalitativ og kvantitativ analyse av primær cilium (PC) biogenese og funksjon.

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Utviklingsbiologi utgave 181 primær cilium menneskeskapte pluripotente stamceller hIPSCer nevrale stamceller og stamceller human cerebral kortikale utvikling nevrale rosetter dorsale forebrain organoider 2D- og 3D hIPSC-baserte modeller for neokortisk utvikling cerebral organoider sonisk pinnsvin helmontert immunostaining optisk rydding lysplatemikroskop
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