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JoVE Journal Neuroscience
Real-Time Fluorescent Measurement of Synaptic Functions in Models of Amyotrophic Lateral Sclerosis
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Real-Time Fluorescent Measurement of Synaptic Functions in Models of Amyotrophic Lateral Sclerosis

Article DOI: 10.3791/62813-v • 08:59 min • July 16th, 2021
July 16th, 2021 •
*1, 1, 1, *1
1Jefferson Weinberg ALS Center, Thomas Jefferson University
* These authors contributed equally

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Summary
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Two related methods are described to visualize subcellular events required for synaptic transmission. These protocols enable the real-time monitoring of the dynamics of presynaptic calcium influx and synaptic vesicle membrane fusion using live-cell imaging of in vitro cultured neurons.

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Real-time Fluorescent Measurement Synaptic Functions Amyotrophic Lateral Sclerosis Experimental Manipulation Disease Causative Protein RNA Therapeutic Compounds Restore Function Reliable Readout Neurons Electrophysiology Neuronal Development Degeneration Rodent Neuronal Cultures Induced Pluripotent Stem Cells Image Acquisition Settings Confocal Imaging Acquisition Software Excitation Power Exposure Time Detector Gain Frame Rate Time Lapse Imaging Dye Bleaching Fluorescence Excitation Dichroic Filter Combinations Emission Filter Combinations Z Drift Perfect Focus Feature Interval Duration Gravity Perfusion
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