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开发一种细胞共培养模型以模拟心脏缺血/体外再灌注
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JoVE Journal Biology
Development of a Cell Co-Culture Model to Mimic Cardiac Ischemia/Reperfusion In Vitro
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开发一种细胞共培养模型以模拟心脏缺血/体外再灌注

DOI:
10.3791/62913-v

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08:05 min

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October 13, 2021

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, , ,
1Department of Anesthesiology,Vanderbilt University Medical Center, 2Anesthesiology,TVHS VA Medical Center, 3Department of Pharmacology,Vanderbilt University

Chapters

  • 00:04Introduction
  • 00:50Experimental Preparation/Plating
  • 02:58Hypoxia/Reoxygenation to Simulate Ischemia/Reperfusion Injury In Vitro
  • 04:28Endpoint Assessment
  • 05:17Results: Effect of Three Types of Culture Inserts and Different Concentrations of Co-Cultured Endothelial Cells on Assessment of the Release of Lactate Dehydrogenase (LDH)
  • 07:36Conclusion

Summary

Automatic Translation

Automatic Translation

空间距离是评估分离内皮和心肌细胞层共培养模型中缺氧/再氧合损伤的关键参数，首次表明优化共培养空间环境对于为测试内皮细胞在心肌细胞保护中的作用提供有利的 体外 模型是必要的。

Tags

Cell Co-cultureCardiac Ischemia/reperfusionIn Vitro ModelCardiomyocyteEndothelial CellSpatial EnvironmentCell-cell InteractionsHypoxia Reperfusion InjuryCell CultureCell Seeding DensityExtracellular Matrix

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