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Determination of Mitochondrial Respiration and Glycolysis in Ex Vivo Retinal Tissue Samples
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Neuroscience
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JoVE Journal Neuroscience
Determination of Mitochondrial Respiration and Glycolysis in Ex Vivo Retinal Tissue Samples

Determination of Mitochondrial Respiration and Glycolysis in Ex Vivo Retinal Tissue Samples

DOI:
10.3791/62914-v

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08:45 min

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August 04, 2021

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, ,
1Neurobiology, Neurodegeneration & Repair Laboratory, National Eye Institute,National Institutes of Health

Chapters

  • 00:04Introduction
  • 01:21Hydrating Sensor Cartridge and Preparation of the Assay Medium
  • 01:55Coating Mesh Inserts of Islet Capture Microplate
  • 02:35Retinal Dissection and Retinal Punch Preparation
  • 04:21Loading the Sensor Cartridge Injection Ports and Calibration
  • 05:09Loading the Islet Capture Plate and Running Assay
  • 06:31Results: Mitochondrial Stress and Glycolytic Rate Assays
  • 07:51Conclusion

Summary

Automatic Translation

Automatic Translation

Described here is a detailed protocol for performing mitochondrial stress assay and glycolytic rate assay in ex vivo retinal tissue samples using a commercial bioanalyzer.

Tags

Mitochondrial RespirationGlycolysisEx VivoSeahorse AnalysisInherited Retinal DegenerationRetinal DissectionMouse RetinaFluorophore ActivationAssay MediumRetinal Punch Preparation

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