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Preparation and Implantation of Electrodes for Electrically Kindling VGAT-Cre Mice to Generate a Model for Temporal Lobe Epilepsy
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Neuroscience
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JoVE Journal Neuroscience
Preparation and Implantation of Electrodes for Electrically Kindling VGAT-Cre Mice to Generate a Model for Temporal Lobe Epilepsy

Preparation and Implantation of Electrodes for Electrically Kindling VGAT-Cre Mice to Generate a Model for Temporal Lobe Epilepsy

DOI:
10.3791/62929-v

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09:29 min

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August 17, 2021

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, , ,
1Department of Pharmacology,University of Virginia, 2UVA Brain Institute,University of Virginia

Chapters

  • 00:04Introduction
  • 00:56Headsets Preparation with Two Bipolar Electrodes
  • 03:24Stereotaxic Electrode Implantation
  • 07:38Results: Study of the Spontaneous Recurring Seizures (SRS) After Electrical Kindling of VGAT-Cre Mice
  • 08:30Conclusion

Summary

Automatic Translation

Automatic Translation

This report describes the methods to generate a model of temporal lobe epilepsy based on the electrical kindling of transgenic VGAT-Cre mice. Kindled VGAT-Cre mice may be useful in determining what causes epilepsy and for screening novel therapies.

Tags

Keywords: VGAT-Cre MiceTemporal Lobe EpilepsyElectrode ImplantationElectrical KindlingEpileptogenesisSpontaneous SeizuresMotor SeizuresElectrodesWireSolderingPinsHemostatForcepsTwisted ElectrodesReference Electrode

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