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Journal / Neuroscience / Generation of Human Motor Units with Functional…
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Neuroscience

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JoVE Journal Neuroscience
Generation of Human Motor Units with Functional Neuromuscular Junctions in Microfluidic Devices
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Generation of Human Motor Units with Functional Neuromuscular Junctions in Microfluidic Devices

Article DOI: 10.3791/62959-v • 10:48 min • September 7th, 2021
September 7th, 2021 •
1,2, 1,2, 1,2, 1,2, 3,4, 3,4, 5, 3,4, 1,2, 1,2, 5, 1,2,6, 1,2
1Department of Neurosciences, Experimental Neurology, and Leuven Brain Institute, KU Leuven - University of Leuven, 2VIB Center for Brain & Disease Research, Laboratory of Neurobiology, , 3VIB Center for Brain & Disease Research, Research Group Molecular Neurobiology, , 4VIB Bio Imaging Core, KU Leuven - University of Leuven, 5Department of Development and Regeneration, Stem Cell and Developmental Biology, KU Leuven - University of Leuven, 6Department of Neurology, University Hospitals Leuven

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Summary
Automatic Translation

We describe a method to generate human motor units in commercially available microfluidic devices by co-culturing human induced pluripotent stem cell-derived motor neurons with human primary mesoangioblast-derived myotubes resulting in the formation of functionally active neuromuscular junctions.

Tags

Generation Human Motor Units Functional Neuromuscular Junctions Microfluidic Devices Research Motor Neurons Health Disease Stem Cell Technology Reproducibility Neuromuscular Diseases Humanized Model Amyotrophic Lateral Sclerosis Motor Unit Sterilization Poly-L-ornithine PLO Solution
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