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JoVE Journal
Developmental Biology

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全卵巢免疫荧光、清除和多光子显微镜用于小鼠发育中卵巢储备的定量3D分析
 
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全卵巢免疫荧光、清除和多光子显微镜用于小鼠发育中卵巢储备的定量3D分析

Article DOI: 10.3791/62972-v 12:36 min September 3rd, 2021
September 3rd, 2021

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Summary

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在这里,我们提出了一种优化的方案,用于对整个卵巢进行成像,以使用全装载免疫染色,多光子显微镜以及3D可视化和分析进行定量和定性分析。该方案适用于高通量、可靠和可重复的处理,适用于毒理学、临床诊断和卵巢功能的基因组测定。

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发育生物学,第175期,
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