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Neuroscience

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JoVE Journal Neuroscience
Characterization of Neuromuscular Junctions in Mice by Combined Confocal and Super-Resolution Microscopy
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Characterization of Neuromuscular Junctions in Mice by Combined Confocal and Super-Resolution Microscopy

Article DOI: 10.3791/63032-v • 11:03 min • December 8th, 2021
December 8th, 2021 •
*1,2, *1, 1,2, 1,2, *1,3, *1,2
1Genethon, 91000, Evry, France, 2Université Paris-Saclay, Univ Evry, Inserm, Genethon, Integrare research unit UMR_S951, 91000, Evry, France, 3Ecole Pratique des Hautes Etudes, PSL Research University, 75014, Paris, France
* These authors contributed equally

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Summary
Automatic Translation

This protocol describes a method for morphometric analysis of neuromuscular junctions by combined confocal and STED microscopy that is used to quantify pathological changes in mouse models of SMA and ColQ-related CMS.

Tags

Neuromuscular Junctions Mice Confocal Microscopy Super-resolution Microscopy Protocol 3D Structure Electron Microscopy Muscle Samples Immunolabeling Morphological Quantification Subcellular Structures Muscle Teasing Immunostainings Image Acquisition Image Analysis Tibialis Anterior Muscles Fiber Bundles Triton X-100 PBS Blocking Solution BSA
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