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Profiling Sensitivity to Targeted Therapies in EGFR-Mutant NSCLC Patient-Derived Organoids
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Profiling Sensitivity to Targeted Therapies in EGFR-Mutant NSCLC Patient-Derived Organoids

Profiling Sensitivity to Targeted Therapies in EGFR-Mutant NSCLC Patient-Derived Organoids

DOI:
10.3791/63039-v

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08:52 min

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November 22, 2021

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, , ,
1Department of Medicine,University of California, San Francisco, 2Helen Diller Family Comprehensive Cancer Center,University of California, San Francisco, 3Department of Cellular and Molecular Pharmacology,University of California, San Francisco

Chapters

  • 00:04Introduction
  • 01:05Generating Single-Cell Suspension
  • 02:38Cell Seeding
  • 04:39Drug Treatment
  • 05:37Readout by Luminescence-Based Survival Assay
  • 06:52Results: The Analysis of Treatment Response of EGFR-Mutant NSCLC Organoid Model to Osimertinib
  • 08:18Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a standardized evaluation of drug sensitivities to targeted signaling inhibitors in NSCLC patient-derived organoid models.

Tags

Keywords: OrganoidsEGFR-mutant NSCLCTargeted TherapiesMatrigelCell CultureDissociationTrypsinizationCentrifugationCell CountingCell Suspension96-well Plate

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