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Cryo-section Dissection of the Adult Subependymal Zone for Accurate and Deep Quantitative Proteome Analysis
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Cryo-section Dissection of the Adult Subependymal Zone for Accurate and Deep Quantitative Proteome Analysis

Cryo-section Dissection of the Adult Subependymal Zone for Accurate and Deep Quantitative Proteome Analysis

DOI:
10.3791/63047-v

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06:24 min

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October 07, 2021

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, ,
1Division of Physiological Genomics, Biomedical Center,Ludwig Maximilian University of Munich, 2Institute for Stem Cell Research,Helmholtz Zentrum München, 3SYNERGY, Excellence Cluster Systems Neurology,University of Munich, 4Department of Clinical Neuroscience,Karolinska Institutet

Chapters

  • 00:04Introduction
  • 00:59Preparation of the Mouse Brain
  • 02:38Sectioning of the Prepared Brain and Free-hand Dissection of Brain Slices
  • 03:48Results: Comparison of Cryo-Section-Dissection with Wholemount and Laser Capture Microdissection for Quantitative Proteome Analysis of the Adult Subependymal Zone
  • 05:34Conclusion

Summary

Automatic Translation

Automatic Translation

Cryo-section-dissection allows fresh, frozen preparation of the largest neurogenic niche in the murine brain for deep quantitative proteome analysis. The method is precise, efficient, and causes minimal tissue perturbation. Therefore, it is ideally suited for studying the molecular microenvironment of this niche, as well as other organs, regions, and species.

Tags

Keywords: Cryo-sectionSubependymal ZoneQuantitative Proteome AnalysisVentricular Neurogenic NicheDissection MethodMass SpectrometryMouse BrainLateral VentriclesCortexCorpus CallosumChoroid PlexusCryostat

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