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Journal / Neuroscience / Cryo-section Dissection of the Adult Subependymal …
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Neuroscience

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JoVE Journal Neuroscience
Cryo-section Dissection of the Adult Subependymal Zone for Accurate and Deep Quantitative Proteome Analysis
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Cryo-section Dissection of the Adult Subependymal Zone for Accurate and Deep Quantitative Proteome Analysis

Article DOI: 10.3791/63047-v • 06:24 min • October 7th, 2021
October 7th, 2021 •
1, 1,2,3, 1,2,4
1Division of Physiological Genomics, Biomedical Center, Ludwig Maximilian University of Munich, 2Institute for Stem Cell Research, Helmholtz Zentrum München, 3SYNERGY, Excellence Cluster Systems Neurology, University of Munich, 4Department of Clinical Neuroscience, Karolinska Institutet

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Summary
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Cryo-section-dissection allows fresh, frozen preparation of the largest neurogenic niche in the murine brain for deep quantitative proteome analysis. The method is precise, efficient, and causes minimal tissue perturbation. Therefore, it is ideally suited for studying the molecular microenvironment of this niche, as well as other organs, regions, and species.

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Cryo-section Dissection Adult Subependymal Zone Quantitative Proteome Analysis Neurogenic Niche Molecular Microenvironment Mass Spectrometry Mouse Brain Training Scalpel Cuts Olfactory Bulb Cortex Lateral Ventricles
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