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JoVE Journal Neuroscience
Kryosektion Dissektion af den voksne subependymale zone til nøjagtig og dyb kvantitativ proteomanalyse
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Kryosektion Dissektion af den voksne subependymale zone til nøjagtig og dyb kvantitativ proteomanalyse

Article DOI: 10.3791/63047-v • 06:24 min • October 7th, 2021
October 7th, 2021 •
1, 1,2,3, 1,2,4
1Division of Physiological Genomics, Biomedical Center, Ludwig Maximilian University of Munich, 2Institute for Stem Cell Research, Helmholtz Zentrum München, 3SYNERGY, Excellence Cluster Systems Neurology, University of Munich, 4Department of Clinical Neuroscience, Karolinska Institutet

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Kryo-sektion-dissektion tillader frisk, frossen forberedelse af den største neurogene niche i murinhjernen til dyb kvantitativ proteomanalyse. Metoden er præcis, effektiv, og forårsager minimal vævforstyrrelse. Derfor er det ideelt egnet til at studere det molekylære mikromiljø af denne niche samt andre organer, regioner og arter.

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Neurovidenskab udgave 176
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