Journal
/
Immunology and Infection
/
Generation of Greater Bacterial Biofilm Biomass using PCR-Plate Deep Well Microplate Devices
JoVE Journal
Immunology and Infection
Author Produced
This content is Free Access.
JoVE Journal Immunology and Infection
Generation of Greater Bacterial Biofilm Biomass using PCR-Plate Deep Well Microplate Devices

Generation of Greater Bacterial Biofilm Biomass using PCR-Plate Deep Well Microplate Devices

DOI:
10.3791/63069-v

•

10:57 min

•

April 22, 2022

•

, , , ,
1Department of Medical Microbiology and Infectious Diseases,University of Manitoba, 2National Microbiology Laboratory,Public Health Agency of Canada

Chapters

  • 00:15Introduction
  • 01:41Days 0-2: Aseptic Culture Preparation for Biofilm Group
  • 02:05Day 2: Preparation and Inoculation of Deep Well Biofilm Plates
  • 03:08Day 3, Part 1: Biofilm Biomass Determination from Device Using CV Staining
  • 04:10Day 3, Part 2: Challenging Biofilms with Antimicrobial to Calculate Their 24 Hour Antimicrobial MBEC Value
  • 04:54Day 4: Recovery of Biofilm Biomass from Pegged Lids and Day 5: Determination of Device MBEC Values
  • 05:44Representative Results
  • 09:07Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol presents methodology to perform biofilm growth and biomass measurements using self-assembled deep well PCR-plate devices for high-throughput 96-well pegged lid static biofilm screening.

Tags

Keywords: Bacterial BiofilmBiofilm BiomassPCR-plate Deep Well MicroplatePeg Lid Biofilm DeviceCrystal Violet StainingAntimicrobial Susceptibility TestingMinimum Biofilm Eradication Concentration (MBEC)

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/63069?article_type=v

Microtiter Dish Biofilm Formation Assay
check_url/63069?article_type=v

In vitro Biofilm Formation in an 8-well Chamber Slide
check_url/63069?article_type=v

Immuno-fluorescence Assay of Leptospiral Surface-exposed Proteins
check_url/63069?article_type=v

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay
check_url/63069?article_type=v

Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks
check_url/63069?article_type=v

Visualization of Bacterial Toxin Induced Responses Using Live Cell Fluorescence Microscopy
check_url/63069?article_type=v

Generation and Multi-phenotypic High-content Screening of Coxiella burnetii Transposon Mutants
check_url/63069?article_type=v

Targeting Biofilm Associated Staphylococcus aureus Using Resazurin Based Drug-susceptibility Assay
check_url/63069?article_type=v

Visualizing the Effects of Sputum on Biofilm Development Using a Chambered Coverglass Model
check_url/63069?article_type=v

Visualization of Biofilm Formation in Candida albicans Using an Automated Microfluidic Device

Read Article