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Immunology and Infection
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JoVE Journal Immunology and Infection
Generation of Greater Bacterial Biofilm Biomass using PCR-Plate Deep Well Microplate Devices
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Generation of Greater Bacterial Biofilm Biomass using PCR-Plate Deep Well Microplate Devices

Article DOI: 10.3791/63069-v • 10:57 min • April 22nd, 2022
April 22nd, 2022 •
1, 1, 1,2, 1,2, 1
1Department of Medical Microbiology and Infectious Diseases, University of Manitoba, 2National Microbiology Laboratory, Public Health Agency of Canada

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Summary
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This protocol presents methodology to perform biofilm growth and biomass measurements using self-assembled deep well PCR-plate devices for high-throughput 96-well pegged lid static biofilm screening.

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PCR-Plate Deep Well Microplate Devices Bacterial Biofilm Peg Lid Biofilm Device Polypropylene Deep Well Biofilm Device Crystal Violet Staining Antimicrobial Susceptibility Testing High Throughput Biofilm Experiments Biofilm Formation Biofilm Biomass Static Biofilms Deep Well Microtiter Plate PCR Plate Peg Surface Area Cost-effective Biofilm Device Protocols For Crystal Violet Staining Minimum Biofilm Eradication Concentration (MBEC) Bacterial Strains
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