High Throughput Biofilm
High Throughput Biofilm - Related Videos
07:09
Use of a High-throughput In Vitro Microfluidic System to Develop Oral Multi-species Biofilms
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Cited by 28 •
2014
The goal of this methods paper is to describe the use of a microfluidic system for the development of multi-species biofilms that contain species typically identified in human supragingival dental plaque. Methods to describe biofilm architecture, biofilm viability, and an approach to harvest biofilm for culture-dependent or culture-independent analyses are highlighted.
08:05
Candida albicans Biofilm Chip (CaBChip) for High-throughput Antifungal Drug Screening
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Cited by 9 •
2012
We have developed a high-density microarray platform consisting of 3D nano-biofilms of C. albicans called CaBChip. The susceptibility profile of drugs tested on a CaBChip is comparable to the conventional 96-well plate model, suggesting that the fungal chip is ideally suited for true high-throughput screening of antifungal drugs.
03:57
Microtiter Dish Biofilm Formation Assay
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Cited by 2243 •
2011
The assay describes a rapid means to measure early biofilm formation in bacteria and fungi. This method uses a microtiter plate as the substratum for microbial biofilm formation, and the biofilm is visualized using crystal violet strain. The assay provides either a qualitative or quantitative assay for early biofilm formation.
09:03
Growth of Mycobacterium tuberculosis Biofilms
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Cited by 79 •
2012
Mycobacterium tuberculosis forms drug tolerant biofilms when cultured in certain conditions. Here we describe methods for culturing M. tuberculosis biofilms and determining the frequency of drug tolerant persisters. These protocols will be useful for further studies into the mechanisms of drug tolerance in M. tuberculosis.
10:17
Methodologies for Studying B. subtilis Biofilms as a Model for Characterizing Small Molecule Biofilm Inhibitors
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Cited by 28 •
2016
This study presents the development of reproducible methodologies to study biofilm inhibitors and their effects on Bacillus subtilis multicellularity.
FAQs
After microorganisms attach to a surface, they produce an extracellular matrix that helps organize the developing community. This matrix is therefore relevant when interpreting measurements of total biomass, viability, or treatment response, because these readouts may reflect both the organisms and the surrounding community structure. Monitoring matrix-associated changes can help compare biofilm development across strains or experimental conditions.
The parallel format supports systematic comparisons among microbial strains, environmental conditions, antimicrobial treatments, and host-relevant factors. Keeping the culture conditions controlled allows researchers to attribute differences in measured biomass, viability, imaging results, or susceptibility more consistently to the variable under study. This design is useful for identifying conditions associated with stronger formation, altered viability, or different treatment responses.
These assay types provide complementary views of a biofilm rather than a single universal outcome. Biomass measurements indicate the amount of community material, viability assays assess living organisms, imaging shows structural or spatial features, and susceptibility assays examine responses to antimicrobial treatment. Considering these readouts together can distinguish changes in accumulation, survival, organization, and treatment response.
A typical workflow begins by culturing microorganisms under controlled conditions in a multiwell plate or another parallelized format. The organisms are allowed to attach and produce their extracellular matrix, after which researchers apply selected measurements such as biomass, viability, imaging, or susceptibility testing. Parallel wells then support direct comparison of strains, conditions, treatments, or host-relevant factors.
Multiwell plates provide a practical format for examining many experimental conditions in parallel while reducing time and sample use. Researchers can organize different strains, environmental conditions, antimicrobial treatments, or host-relevant factors across wells and evaluate them with consistent assay types. This arrangement supports systematic screening and comparison when investigating persistent infection-associated communities.
In immunology and infection research, high-throughput biofilm analysis helps investigate communities associated with persistent infections and evaluate factors that may influence their behavior. Host-relevant conditions can be compared alongside microbial and treatment variables, while susceptibility assays provide information about antimicrobial responses. The resulting comparisons can guide studies of treatment tolerance and broader infection control strategies.