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Characterization of Blood Outgrowth Endothelial Cells (BOEC) from Porcine Peripheral Blood
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JoVE Journal Bioengineering
Characterization of Blood Outgrowth Endothelial Cells (BOEC) from Porcine Peripheral Blood

Characterization of Blood Outgrowth Endothelial Cells (BOEC) from Porcine Peripheral Blood

DOI:
10.3791/63285-v

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08:02 min

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January 06, 2022

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, , , , ,
1Department of Biomedical Engineering,Medical College of Wisconsin & Marquette University, 2Division of Engineering,Mayo Clinic, 3Department of Physiology & Biomedical Engineering,Mayo Clinic, 4Department of Cardiovascular Medicine,Mayo Clinic

Chapters

  • 00:04Introduction
  • 00:37Collection of Porcine Peripheral Blood
  • 02:36Isolation of Mononuclear Cells
  • 03:33Washing and Plating of Cells
  • 05:16Cell Culture
  • 05:50Results: Morphological Differentiation, Organization and Characterization of Blood Outgrowth Endothelial Cells (BOECs)
  • 07:27Conclusion

Tags

Keywords: Blood Outgrowth Endothelial CellsBOECPorcinePeripheral BloodCell TherapyDisease ModelingHeparinFemoral VeinFemoral ArteryDensity GradientCentrifugationCell Culture

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