Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Locomotor Assessment of 6-Hydroxydopamine-induced …
JoVE Journal
Neuroscience

A subscription to JoVE is required to view this content.

JoVE Journal Neuroscience
Locomotor Assessment of 6-Hydroxydopamine-induced Adult Zebrafish-based Parkinson's Disease Model
Video Acquisition and Preparing the Videos for the MouseWalker (MW) Tracking Software (Video) | JoVE
Journal (Neuroscience)
Video Acquisition and Preparing the Videos for the MouseWalker (MW) Tracking Software (Video) | JoVE
Whole Cell Patch Clamp for Investigating the Mechanisms of Infrared Neural Stimulation
Journal (Neuroscience)
Whole Cell Patch Clamp for Investigating the Mechanisms of Infrared Neural Stimulation
 

Locomotor Assessment of 6-Hydroxydopamine-induced Adult Zebrafish-based Parkinson's Disease Model

Article DOI: 10.3791/63355-v • 07:32 min • December 28th, 2021
December 28th, 2021 •
1, 1, 1,2, 1, 1, 2, 1
1Collaborative Drug Discovery Research (CDDR) Group and Brain Degeneration and Therapeutics Group, Faculty of Pharmacy, Universiti Teknologi MARA (UiTM), Cawangan Selangor, Kampus Puncak Alam, 2Department of Medicine, Faculty of Medicine, Universiti Malaya

Chapters

Summary
Automatic Translation

The present protocol describes the intracerebroventricular (ICV) injection of adult zebrafish with neurotoxic 6-hydroxydopamine (6-OHDA) at the ventral diencephalon (Dn) and the assessment of the impairment and subsequent recovery of swimming behavior postlesion by using the open tank test, which is accompanied by analysis using a video tracking software.

Tags

Locomotor Assessment 6-Hydroxydopamine-induced Parkinson's Disease Model Zebrafish Motor Impairment Neurotoxin Injection Dopaminergic Neurons Ablation Functional Effects Setup Neuroregeneration Mechanisms Cell Replacement Treatment Strategies Fish Maintenance Water Tank Sea Salt Temperature Control Light-dark Photoperiod Feeding Regimen Tricaine Methanesulfonate Stock Solution Anesthesia Solution 6-hydroxydopamine Preparation
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter