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原代人鼻上皮细胞：精准医学背景下的生物样本库

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Biology

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JoVE Journal Biology

Primary Human Nasal Epithelial Cells: Biobanking in the Context of Precision Medicine

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原代人鼻上皮细胞：精准医学背景下的生物样本库

DOI:

10.3791/63409-v

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08:35 min

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April 22, 2022

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Mairead Kelly², Elise Dreano², Aurelie Hatton², Agathe Lepissier², Anita Golec², Isabelle Sermet-Gaudelus^2,3, Iwona Pranke^2,3

¹Institut Necker Enfants Malades, ²Université de Paris, ³Centre de Référence Maladies Rares Mucoviscidose et Maladies apparentées,Assistance Publique Hôpitaux de Paris

Chapters

00:04Introduction
00:56Amplification and Passaging of Human Nasal Epithelial (HNE) Cells
02:26Cryopreservation of Primary Nasal Epithelial Cells
03:06Thawing Frozen Amplified HNE Cells
04:38Differentiation of HNE Cells at the Air-Liquid Interface
05:23Results: Analysis of Electrophysiological Properties of Differentiated HNE Cells and CFTR-Related Chloride Secretion Correction
07:59Conclusion

Summary

Automatic Translation

在这里，我们描述了原代人鼻上皮（HNE）细胞在气液界面处的分离，扩增和分化，以及允许成功冷冻和解冻扩增的HNE的生物样本库方案。该方案分析了不同调节剂处理下分化HNE细胞的电生理特性和CFTR相关氯离子分泌校正。

Tags

Primary Nasal Epithelial Cells Biobanking Precision Medicine Lung Epithelium Patient-derived Cultures Personalized Medicine CFTR Activity Cystic Fibrosis Therapy Amplification Medium Cryopreservation Cell Culture Cell Counting Freezing Medium Cryofreezing Nitrogen Storage

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