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Standardisering og vedlikehold av 3D-hundeorpatiske og intestinale organoidkulturer for bruk i biomedisinsk forskning
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Standardisering og vedlikehold av 3D-hundeorpatiske og intestinale organoidkulturer for bruk i biomedisinsk forskning

Article DOI: 10.3791/63515-v • 08:39 min • January 31st, 2022
January 31st, 2022 •
1, 1, 2, 3, 2, 2, 4, 4, 1,3, 2,3
1Department of Biomedical Sciences, College of Veterinary Medicine, Iowa State University, 2Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University, 33D Health Solutions Inc., 4Department of Molecular Virology and Microbiology, Baylor College of Medicine

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Eksperimentelle metoder for å høste voksne stamceller fra hundens tarm- og levervev for å etablere 3D organoidkulturer beskrives. Videre diskuteres laboratorieteknikkene for å sikre konsistent vekst og gi standard driftsprosedyrer for høsting, biobank og gjenopplive hundens tarm- og leverorganoidkulturer.

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Medisin Utgave 179 Organoid hund hund stamcelle lever tarm En helse standardisering
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