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Freeze-Fracture Electron Microscopy for Extracellular Vesicle Analysis
 

Freeze-Fracture Electron Microscopy for Extracellular Vesicle Analysis

Article DOI: 10.3791/63550-v
September 16th, 2022

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Summary September 16th, 2022

We present a protocol for the isolation and freeze-fracturing of extracellular vesicles (EVs) originating from cancerous urothelial cells. The freeze-fracture technique revealed the EVs' diameter and shape and-as a unique feature-the internal organization of the EV membranes. These are of immense importance in understanding how EVs interact with the recipient membranes.

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