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Frysefraktur elektronmikroskopi for ekstracellulær vesicleanalyse
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Frysefraktur elektronmikroskopi for ekstracellulær vesicleanalyse

Article DOI: 10.3791/63550-v • 11:30 min • September 16th, 2022
September 16th, 2022 •
1, 1, 1, 1
1Faculty of Medicine, Institute of Cell Biology, University of Ljubljana

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Vi presenterer en protokoll for isolering og frysefraktur av ekstracellulære vesyrer (ELBILER) som stammer fra kreftfremkallende urotelceller. Frysefrakturteknikken avslørte ELBIL-enes diameter og form og-som en unik funksjon - den interne organiseringen av EV-membranene. Disse er av enorm betydning for å forstå hvordan elbiler samhandler med mottakermembranene.

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